Synthesis, characterization and biological evaluation of strontium/magnesium-co-substituted hydroxyapatite

Abstract

The present study aims to investigate the contribution of two biologically important cations, Mg(2+) and Sr(2+), when co-substituted into the structure of hydroxyapatite (Ca10(PO4)6(OH)2, HA). The substituted samples were synthesized by a hydrothermal method that involved the addition of Mg(2+) and Sr(2+) containing precursors to partially replace Ca(2+) in the apatite structure. Four co-substituted HA samples with different concentrations of Mg(2+) and Sr(2+) ((Mg + Sr)/(Mg + Sr + Ca) = 30%) were investigated, and they were compared with pure HA. Experimental results showed that only a limited amount of Mg (Mg/(Mg + Ca + Sr) < 14%) could successfully substitute for Ca in HA. In addition, Mg substitution resulted in reduced crystallinity, thermal stability and lattice parameters of HA. In contrast, Sr could fully substitute for Ca. Furthermore, the addition of Sr increased the lattice parameters of HA. Here, we obtained the cation leach liquor by immersing the prepared samples in a culture medium for cell experiments. The in vitro study showed that 10Mg20Sr promoted better MG63 cell attachment, proliferation and differentiation than HA. Thus, the presence of an appropriate proportion of Mg and Sr could play a significant role in the increased biocompatibility of HA.

Keywords: Hydroxyapatite; biocompatibility; cation leach liquor; co-substitution; crystallinity.