Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Feb 26;351(6276):943-9.
doi: 10.1126/science.aad5309.

The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation

T Robert  1 , A Nore  1 , C Brun  1 , C Maffre  1 , B Crimi  1 , H-M Bourbon  2 , B de Massy  3
Affiliations

The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation

T Robert et al. Science. .

Erratum in

Abstract

Meiotic recombination is induced by the formation of DNA double-strand breaks (DSBs) catalyzed by SPO11, the ortholog of subunit A of TopoVI DNA topoisomerase (TopoVIA). TopoVI activity requires the interaction between A and B subunits. We identified a conserved family of plant and animal proteins [the TOPOVIB-Like (TOPOVIBL) family] that share strong structural similarity to the TopoVIB subunit of TopoVI DNA topoisomerase. We further characterize the meiotic recombination proteins Rec102 (Saccharomyces cerevisiae), Rec6 (Schizosaccharomyces pombe), and MEI-P22 (Drosophila melanogaster) as homologs to the transducer domain of TopoVIB. We demonstrate that the mouse TOPOVIBL protein interacts and forms a complex with SPO11 and is required for meiotic DSB formation. We conclude that meiotic DSBs are catalyzed by a complex involving SPO11 and TOPOVIBL.

PubMed Disclaimer

Comment in

  • DNA. Breaking DNA.
    Bouuaert CC, Keeney S. Bouuaert CC, et al. Science. 2016 Feb 26;351(6276):916-7. doi: 10.1126/science.aaf2509. Science. 2016. PMID: 26917753 No abstract available.

Publication types

MeSH terms