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. 2016 Jun;179(3):398-414.
doi: 10.1007/s12010-016-2002-2. Epub 2016 Feb 27.

Challenges and Opportunities in the Development of Aptamers for TNFα

Claudia Nübel  1 , Bettina Appel  1 , Ingeborg Hospach  2   3 , Michaela Mai  2 , Nadejda Krasteva  2 , Gabriele Nelles  2 , Lothar Petruschka  4 , Sabine Müller  5
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Challenges and Opportunities in the Development of Aptamers for TNFα

Claudia Nübel et al. Appl Biochem Biotechnol. 2016 Jun.

Abstract

RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not the only crucial parameter for efficacy and functionality of those aptamers. Instead, the sensitive equilibrium of the monomeric and homotrimeric form of soluble TNFα decides on aptamer binding. Our results show that the field of application and the source of TNFα have to be carefully defined before selection of aptamer sequences.

Keywords: Aptamer; Biotin linker; Cytokine; ELISA; In vitro selection; QCM; Rational design; SPR; TNFα.

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