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. 2015 Jan-Mar;15(1):33-8.
doi: 10.4103/0972-4052.155040.

A comparative study to evaluate the osteoblastic cell behavior of two nano coated titanium surfaces with NAFION stabilized the membrane

Sanjna Nayar  1 , Sanket Chakraverty  2
Affiliations

A comparative study to evaluate the osteoblastic cell behavior of two nano coated titanium surfaces with NAFION stabilized the membrane

Sanjna Nayar et al. J Indian Prosthodont Soc. 2015 Jan-Mar.

Abstract

Aim: The aim of the study was to comparatively analyze the in vitro cell adhesion between nano coated titanium dioxide, and calcium hydroxyapatite (HA) coated titanium samples.

Materials and methods: Nano coated titanium dioxide, and calcium HA were coated onto the titanium samples by drop casting with NAFION membrane and cell culture was done by seeding human osteoblastic sarcoma cells on the coated samples.

Results and conclusion: There was marked cell adhesion seen in the samples coated by titanium dioxide nano particles and more cells spreading as compared to calcium HA nano particles.

Keywords: Calcium hydroxyapatite; NAFION membrane; cyclic voltammetry; elemental analysis; fluorescence microscopy; human osteoblastic sarcoma cells; nano coated titanium dioxide.

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Conflict of interest statement

Conflict of Interest: None.

Figures

Figure 1
Figure 1
Simple machined samples
Figure 2
Figure 2
The architecture of NAFION membrane
Figure 3
Figure 3
The coated nano titanium dioxide sample and the scanning electron microscopy of the nano titanium dioxide
Figure 4
Figure 4
Even after 100 continuous cycles the nano TiO2 is held tight on the titanium disk with the help of the NAFION membrane
Figure 5
Figure 5
Nano calcium hydroxyapatite coated sample and scanning electron microscopy analysis of the sample
Figure 6
Figure 6
Energy dispersive spectroscopy for nano calcium hydroxyapatite particles
Figure 7
Figure 7
Scanning electron microscopy analysis showing osteoblastic cell adhesion for control
Figure 8
Figure 8
Scanning electron microscopy analysis shows osteoblastic cell adhesion for nano calcium hydroxyapatite
Figure 9
Figure 9
Scanning electron microscopy analysis shows osteoblastic cell adhesion for nano titanium dioxide
Figure 10
Figure 10
Fluorescence spectroscopy as seen in (a) control, (b) nano coated calcium hydroxide apatite and (c) nano coated titanium dioxide
See this image and copyright information in PMC

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