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. 2015 Oct;11(Suppl 3):S496-500.
doi: 10.4103/0973-1296.168981.

Modulatory Role of Shorea robusta Bark on Glucose-metabolizing Enzymes in Diethylnitrosamine Induced Hepatocellular Carcinoma in Rats

A Kalaiselvan  1 , T Anand  1 , K Gokulakrishnan  2 , M C Kamaraj  1 , S Velavan  3
Affiliations

Modulatory Role of Shorea robusta Bark on Glucose-metabolizing Enzymes in Diethylnitrosamine Induced Hepatocellular Carcinoma in Rats

A Kalaiselvan et al. Pharmacogn Mag. 2015 Oct.

Abstract

Introduction: The modulations of glucose-metabolizing enzyme activities play a vital rolein the depletion of energy metabolism and leads to inhibition of cancer growth.

Objective: To find the effect of shorearobusta bark extract on glucose-metbolizing enzymes in diethylnitrosamine (DEN) induced hepatocellular carcinoma rats.

Materials and methods: Biochemical evaluation of glucose metabolizing enzyme were done in before and after shorearobusta bark extract (500mg/kg) treatment in DEN induced rats.

Results: A significant increasein the activities of the key glycolytic enzymes viz., hexokinase and phosphoglucoisomerase, with a significant decrease in the gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatasewere observed in HCC bearing rats, when compared with the control. Administration of shorearobusta extract caused a significant decrease in theactivities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values.

Conclusion: The current findings suggest that the S. robusta extract has a definite modulating role on the key enzymes ofglucose-metabolism in HCC. The modulatory effect may be due to the phytoactive constituents present in the extract of S. robusta.

Summary: Administration of shorea robusta bark extract caused a significant decrease in the activities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values. The S. robusta extract has modulatory activity on the carbohydrate metabolism in DEN-induced HCC bearing rats through a mechanism that which does not provoke any acute biochemical disturbances in the metabolic pathways of glycolysis and gluconeogenesis. The modulatory effect of S. robusta extract may be attributed to the presence of active compounds such as polyphenols and flavonoids. Abbreviations used: HCC: Hepatocellular Carcinoma, SRBE: Shorearobusta bark extract; HEX: Hexokinase; PGI: Phosphoglucoisomerase; DEN: Diethylnitrosamine.

Keywords: Diethylnitrosamine; Shorea robusta; glucose-metabolizing enzymes; hepatocellular carcinoma.

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Figures

Figure 1
Figure 1
Effect of Shorea robusta bark on liver hexokinase in control and experimental rats. aP < 0.001 significantly different compared with Groups I and II control animals. bP < 0.001 significantly different compared with Group III animals
Figure 2
Figure 2
Effect of Shorea robusta bark on liver phosphoglucoisomerase in control and experimental rats. aP < 0.001 significantly different compared with Groups I and II control animals. bP < 0.001 significantly different compared with Group III animals
Figure 3
Figure 3
Effect of Shorea robusta bark on liver glucose-6-phosphatease and fructose-1,6-diphosphatase in control and experimental rats. aP < 0.001 significantly different compared with Groups I and II control animals. bP < 0.001 significantly different compared with Group III animals
None
A. Kalaiselvan
None
T. Anand
None
K. Gokulakrishnan
None
M. C. Kamaraj
None
S. Velavan
None
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