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Randomized Controlled Trial
. 2016 Feb 29:13:7.
doi: 10.1186/s12970-016-0119-x. eCollection 2016.

Effects of combined β-hydroxy-β-methylbutyrate (HMB) and whey protein ingestion on symptoms of eccentric exercise-induced muscle damage

Minayuki Shirato #  1 , Yosuke Tsuchiya #  2 , Teruyuki Sato  3 , Saki Hamano  2 , Takeshi Gushiken  1 , Naoto Kimura  1 , Eisuke Ochi  4
Affiliations
Randomized Controlled Trial

Effects of combined β-hydroxy-β-methylbutyrate (HMB) and whey protein ingestion on symptoms of eccentric exercise-induced muscle damage

Minayuki Shirato et al. J Int Soc Sports Nutr. .

Abstract

Background: The purpose of this study was to examine the effects of combined β-hydroxy-β-methylbutyrate (HMB) and whey protein ingestion on muscle strength and damage following a single bout of eccentric exercise.

Methods: Eighteen untrained male subjects were assigned to HMB and Whey protein (HMB + Whey; 3 g/day HMB and 36.6 g/day whey protein, n = 6), HMB (3 g/day, n = 6), or whey protein (36.6 g/day, n = 6) groups. Ingestion commenced 7 days before non-dominant elbow flexor eccentric exercise (30 deg/sec, 6 reps × 7 sets) and continued until 4 days post-exercise. The maximal isometric strength, muscle soreness, plasma creatine kinase (CK), lactate dehydrogenase (LDH) were assessed pre-exercise, and at 1, 2, 3, and 5 days after exercise.

Results: The change scores of maximal isometric strength significantly decreased at day 1, 2, and 5 in the whey protein group compared to pre value and that in HMB + Whey protein and HMB groups decreased at day 1 and 5. The muscle soreness significantly increased in the whey and HMB + Whey protein groups at day 3 compared to pre value (p < 0.05). CK and LDH significantly increased (time effect: p < 0.05) after exercise. However, all data were not significant difference among the groups.

Conclusions: These results suggest that ingestion of combined HMB and whey protein does not have a role to inhibit muscle strength loss and soreness, and decrease in muscle damage markers after eccentric exercise in comparison with HMB and whey protein alone.

Keywords: Amino acids; Lengthening; Muscle soreness; Muscle strength.

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Figures

Fig. 1
Fig. 1
Experimental protocol. Ingestion groups: HMB (3 g/day) and whey protein (36.6 g/day) group, HMB (3 g/day) group, and whey protein (36.6 g/day) group. Supplements were taken for 7 days before and 4 days after eccentric exercise (supplementation for 12 days). Blood was analyzed before and at 1, 2, 3, and 5 days post-exercise. CK: creatine kinase, LDH: lactate dehydrogenase, Isometric strength: maximal voluntary isometric contraction torque of elbow in flexed position (90°), Muscle soreness: 100-mm visual analogue scale for biceps brachii
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