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Case Reports
. 2016 Feb 29:11:22.
doi: 10.1186/s40793-016-0139-9. eCollection 2016.

Genome sequences of Knoxdaviesia capensis and K. proteae (Fungi: Ascomycota) from Protea trees in South Africa

Janneke Aylward  1 , Emma T Steenkamp  2 , Léanne L Dreyer  1 , Francois Roets  3 , Brenda D Wingfield  4 , Michael J Wingfield  2
Affiliations
Case Reports

Genome sequences of Knoxdaviesia capensis and K. proteae (Fungi: Ascomycota) from Protea trees in South Africa

Janneke Aylward et al. Stand Genomic Sci. .

Abstract

Two closely related ophiostomatoid fungi, Knoxdaviesia capensis and K. proteae, inhabit the fruiting structures of certain Protea species indigenous to southern Africa. Although K. capensis occurs in several Protea hosts, K. proteae is confined to P. repens. In this study, the genomes of K. capensis CBS139037 and K. proteae CBS140089 are determined. The genome of K. capensis consists of 35,537,816 bp assembled into 29 scaffolds and 7940 predicted protein-coding genes of which 6192 (77.98 %) could be functionally classified. K. proteae has a similar genome size of 35,489,142 bp that is comprised of 133 scaffolds. A total of 8173 protein-coding genes were predicted for K. proteae and 6093 (74.55 %) of these have functional annotations. The GC-content of both genomes is 52.8 %.

Keywords: Gondwanamycetaceae; Knoxdaviesia; Microascales; Ophiostomatoid fungi; Protea.

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Figures

Fig. 1
Fig. 1
Maximum Likelihood tree illustrating the phylogenetic position of K. capensis and K. proteae in the Gondwanamycetaceae (grey block). The Protea-associated species are shaded red and the two isolates for which genome sequences were determined are indicated with a box. The sequences of the Internal Transcribed Spacer (ITS) region (available from GenBank®, accession numbers in brackets following isolate numbers) were aligned in MAFFT 7 [55]. The phylogeny was calculated in MEGA6 [56] using the Tamura-Nei substitution model [57], 1000 bootstrap replicates and Ceratocystis fimbriata (Ceratocystidaceae) as an outgroup
Fig. 2
Fig. 2
Sexual sporing structures of the two Knoxdaviesia species sequenced in this study. K. capensis (a) and K. proteae (b) were sampled from Protea longifolia and P. repens flowers, respectively. Scale bars = 1 mm
See this image and copyright information in PMC

References

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