Massilibacterium senegalense gen. nov., sp. nov., a new bacterial genus isolated from the human gut

Abstract

Massilibacterium senegalense gen. nov., sp. nov., strain mt8(T), is the type strain of Massilibacterium gen. nov., a new genus within the Bacillaceae family. This Gram-negative facultative anaerobic rod was isolated from the gut microbiota of a severely malnourished boy. Its phenotypic description is hereby presented with a complete annotation of its genome sequence. This genome is 5 697 950 bp long and contains 5615 protein-coding genes and 178 RNA genes, among which are 40 rRNA genes.

Keywords: Culturomics; Massilibacterium senegalense; genome; gut microbiota; taxonogenomics.

Fig. 1

Phylogenetic tree highlighting the position of Massilibacterium senegalense strain mt8^T relative to other close strains. The respective GenBank accession numbers for 16S rRNA genes are indicated in parenthesis. Sequences were aligned using CLUSTALW, and phylogenetic inferences were obtained using the maximum-likelihood method within the MEGA6. Numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis 1,000 times to generate a majority consensus tree. Paenibacillus polymyxa strain KCTC3717 was used as an outgroup. The scale bar represents a 1% nucleotide sequence divergence.

Fig. 2

Reference mass spectrum from Massilibacterium senegalense strain mt8^T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.

Fig. 3

Gel view comparing Massilibacterium senegalense strain mt8^T to other species within the Bacillaceae family. The gel view displays the raw spectra of loaded spectrum files arranged in a pseudo-gel like look. The x-axis records the m/z value. The left y-axis displays the running spectrum number originating from subsequent spectra loading. The peak intensity is expressed by a Gray scale scheme code. The color bar and the right y-axis indicate the link between the color in which a peak is displayed and the peak intensity in arbitrary units. Displayed species are indicated on the left.

Fig. 4

Gram staining of Massilibacterium senegalense strain mt8^T.

Fig. 5

Transmission electron microscopy of Massilibacterium senegalense strain mt8^T using Tecnai G20 transmission electron microscope (FEI Company). Scale bar = 500 nm.

Fig. 6

Graphical circular map of chromosome. From outside to center: genes on forward strain coloured by COGs categories (only gene assigned to COGs), RNA genes (tRNAs green, rRNAs red), G+C content and G+C skew.

Fig. 7

Distribution of functional classes of predicted genes according to clusters of orthologous groups of protein.