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. 1989 Sep;67(3):343-6.
doi: 10.1111/j.1365-2672.1989.tb02503.x.

Membrane filtration differentiation of E. coli from coliforms in the examination of water

A Mates  1 , M Shaffer
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Membrane filtration differentiation of E. coli from coliforms in the examination of water

A Mates et al. J Appl Bacteriol. 1989 Sep.

Abstract

A membrane filter-Endo agar method for enumerating Escherichia coli as distinct from other coliforms in drinking water was developed. Membranes containing coliform colonies are transferred to nutrient agar containing 4-methyl umbelliferyl-beta-D-glucuronide (MUG) and incubated at 35 degrees C for 4 h. The MUG is hydrolyzed by the glucuronidase of E. coli and the fluorogenic product is visualized. The method recovered 98% of E. coli without false positives and is proposed as an additional test in routine water examination for the detection of pollution.

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