Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody
- PMID: 2693617
- DOI: 10.1016/0022-510x(89)90223-2
Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody
Abstract
The value of analysing dystrophin on Western blots of skeletal muscle for the differential diagnosis of Xp21 muscular dystrophies is now fairly well established. Here we describe a sensitive system based on monoclonal antibodies to dystrophin. The specificity of the antibodies was established and experiments were undertaken to identify the source of dystrophin-related protein bands which were detected on blots of normal skeletal muscle. These investigations formed a necessary preliminary study to the application of the assay to samples of muscle obtained at biopsy from patients with Duchenne and Becker muscular dystrophy.
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