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. 2016 Jan-Apr;6(1):2-9.
doi: 10.1016/j.jobcr.2015.12.005. Epub 2015 Dec 24.

Surface properties and early murine pre-osteoblastic cell responses of phosphoric acid modified titanium surface

Thanaphum Osathanon  1 , Chenphop Sawangmake  2 , Nanticha Ruangchainicom  3 , Pavitra Wutikornwipak  3 , Panisa Kantukiti  3 , Nunthawan Nowwarote  3 , Prasit Pavasant  1
Affiliations

Surface properties and early murine pre-osteoblastic cell responses of phosphoric acid modified titanium surface

Thanaphum Osathanon et al. J Oral Biol Craniofac Res. 2016 Jan-Apr.

Abstract

Aims: The present study investigated the surface properties and murine pre-osteoblast cell (MC3T3-E1) responses of phosphoric acid (H3PO4) treated commercially pure titanium.

Methods: Titanium discs were treated with various concentration of H3PO4 (5%, 10%, and 20%; v/v) at 90 °C for 30 min. Surface properties were evaluated by profilometer, contact angle meter, and scanning electron microscopy (SEM) with energy dispersive X-rays. MC3T3-E1 attachment and spreading were evaluated by SEM and phalloidin immunohistochemistry staining.

Results: Surface roughness and wettability were not statistically difference among all experimental and control groups. Phosphate and oxygen were detected on H3PO4 treated surfaces. At 20 min, cell attachment was significantly higher in 10% and 20% H3PO4 treated groups compared to the control. Cells exhibited orientated-cytoskeleton fibers on 20% H3PO4 modified titanium surface. Though, there was no difference in cell spreading stage among all treatment groups.

Conclusion: H3PO4 treatment on titanium may influence early cell response, particularly on attachment and spreading.

Keywords: Cell attachment; Cell spreading; Osteoblast; Surface modification; Titanium.

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Figures

Fig. 1
Fig. 1
Representative SEM micrographs illustrated surface morphology of the control (A), 5% (B), 10% (C), and 20% (D) H3PO4 treated titanium surfaces (2,000X magnification).
Fig. 2
Fig. 2
Graphs showed the number of cells per filed normalized to the control at 20 min (A) and 1 h (B). Asterisks indicated the statistical significant difference compared to the control.
Fig. 3
Fig. 3
Representative SEM micrographs illustrated cell morphology on different titanium surface modifications (350× magnification).
Fig. 4
Fig. 4
Representative SEM micrographs illustrated cell morphology on different titanium surface modifications (2000× magnification).
Fig. 5
Fig. 5
Graphs showed the percentage of cell spreading stages as evaluated by SEM at 1 h after cells seeding on modified surfaces.
Fig. 6
Fig. 6
Representative images illustrated the F-actin orientation at different time points after cells seeding on different surface modifications. Bars indicated 10 μm.
See this image and copyright information in PMC

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