Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Oct;219(1-2):58-64.
doi: 10.1007/BF00261157.

Yeast mutants with enhanced ability to secrete human lysozyme: isolation and identification of a protease-deficient mutant

K Suzuki  1 , K Ichikawa, Y Jigami
Affiliations

Yeast mutants with enhanced ability to secrete human lysozyme: isolation and identification of a protease-deficient mutant

K Suzuki et al. Mol Gen Genet. 1989 Oct.

Abstract

Yeast mutant strains which secrete large amounts of human lysozyme were screened using an agar medium containing bacterial cells. Nine mutants secreted over 10 times more lysozyme than the wild-type parent strain. The mRNA levels for lysozyme in the mutants were not higher than that of the wild-type strain. Three of the mutant strains were deficient in carboxypeptidase Y activity. It was found that the protease deficiency was caused by a deficiency in conversion of proenzyme to mature enzyme in ssl1 mutant cells. The ssl1 gene was found to be closely linked to the centromere and determine both the efficiency of secretion of lysozyme and the processing of carboxypeptidase Y.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Curr Genet. 1986;10 (5):353-7 - PubMed
    1. Anal Biochem. 1983 Jan;128(1):77-85 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Bacteriol. 1975 Sep;123(3):1150-6 - PubMed
    1. Genetics. 1988 Jul;119(3):499-506 - PubMed

LinkOut - more resources