Draft Genome Sequence of a Strictly Anaerobic Dichloromethane-Degrading Bacterium

Sara Kleindienst¹, Steven A Higgins², Despina Tsementzi³, Konstantinos T Konstantinidis⁴, E Erin Mack⁵, Frank E Löffler⁶

Affiliations

¹ University of Tennessee and Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA sara.kleindienst@uni-tuebingen.de frank.loeffler@utk.edu.
² Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA.
³ School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
⁴ School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.
⁵ Corporate Remediation Group, E. I. DuPont de Nemours and Company, Newark, Delaware, USA.
⁶ University of Tennessee and Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee, USA sara.kleindienst@uni-tuebingen.de frank.loeffler@utk.edu.

PMID: 26941136
PMCID: PMC4777747
DOI: 10.1128/genomeA.00037-16

Draft Genome Sequence of a Strictly Anaerobic Dichloromethane-Degrading Bacterium

Sara Kleindienst et al. Genome Announc. 2016.

. 2016 Mar 3;4(2):e00037-16.

doi: 10.1128/genomeA.00037-16.

Authors

Sara Kleindienst¹, Steven A Higgins², Despina Tsementzi³, Konstantinos T Konstantinidis⁴, E Erin Mack⁵, Frank E Löffler⁶

Affiliations

¹ University of Tennessee and Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA sara.kleindienst@uni-tuebingen.de frank.loeffler@utk.edu.
² Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA.
³ School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
⁴ School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.
⁵ Corporate Remediation Group, E. I. DuPont de Nemours and Company, Newark, Delaware, USA.
⁶ University of Tennessee and Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee, USA sara.kleindienst@uni-tuebingen.de frank.loeffler@utk.edu.

PMID: 26941136
PMCID: PMC4777747
DOI: 10.1128/genomeA.00037-16

Abstract

An anaerobic, dichloromethane-degrading bacterium affiliated with novel Peptococcaceae was maintained in a microbial consortium. The organism originated from pristine freshwater sediment collected from Rio Mameyes in Luquillo, Puerto Rico, in October 2009 (latitude 18°21'43.9″, longitude -65°46'8.4″). The draft genome sequence is 2.1 Mb and has a G+C content of 43.5%.

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References

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1. Egli C, Tschan T, Scholtz R, Cook AM, Leisinger T. 1988. Transformation of tetrachloromethane to dichloromethane and carbon dioxide by Acetobacterium woodii. Appl Environ Microbiol 54:2819–2824. - PMC - PubMed
1. Mägli A, Rainey FA, Leisinger T. 1995. Acetogenesis from dichloromethane by a two-component mixed culture comprising a novel bacterium. Appl Environ Microbiol 61:2943–2949. - PMC - PubMed
1. Freedman DL, Gossett JM. 1991. Biodegradation of dichloromethane and its utilization as a growth substrate under methanogenic conditions. Appl Environ Microbiol 57:2847–2857. - PMC - PubMed
1. Freedman DL, Smith CR, Noguera DR. 1997. Dichloromethane biodegradation under nitrate-reducing conditions. Water Environ Res 69:115–122. doi:10.2175/106143097X125245. - DOI

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R01 ES024294/ES/NIEHS NIH HHS/United States

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Draft Genome Sequence of a Strictly Anaerobic Dichloromethane-Degrading Bacterium

Affiliations

Draft Genome Sequence of a Strictly Anaerobic Dichloromethane-Degrading Bacterium

Authors

Affiliations

Abstract

References

Grants and funding