doi: 10.1073/pnas.74.9.3859.
Separation and infectivity of circular and linear forms of potato spindle tuber viroid
- PMID: 269437
- PMCID: PMC431760
- DOI: 10.1073/pnas.74.9.3859
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Separation and infectivity of circular and linear forms of potato spindle tuber viroid
Proc Natl Acad Sci U S A.
1977 Sep.
Abstract
Potato spindle tuber viroid can be separated into two fractions by polyacrylamide gelelectrophoresis in the presence of formamide and urea. One fraction contains predominantly circular molecules; the second fraction contains almost exclusively linear molecules. The purity of the fractions was estimated by electron microscopy of formaldehyde-denatured molecules. The length distributions of denatured circular and linear molecules were determined. Both circular and linear molecules were found to be infectious.
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