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. 1977 Sep;74(9):3937-41.
doi: 10.1073/pnas.74.9.3937.

Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids

R E Fournier, F H Ruddle

Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids

R E Fournier et al. Proc Natl Acad Sci U S A. 1977 Sep.

Abstract

Trispecific microcell hybrids were prepared by transferring limited numbers of chromosomes from a human/mouse gene-transfer cell line to a Chinese hamster recipient line. The donor cells employed were murine L-cells that stably expressed the human form of the enzyme hypoxanthine phosphoribosyltransferase. Karyotypic, zymographic, and back-selection tests of the resulting human/mouse/Chinese hamster microcell hybrids provided strong genetic evidence for a stable association of the human transgenome with host murine chromosomes in stable gene-transfer cell lines. This association, which may represent physical integration of the transgenome into the host cell genome, occurred at multiple chromosomal sites.

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References

    1. Science. 1964 Aug 14;145(3633):709-10 - PubMed
    1. Exp Cell Res. 1977 Mar 1;105(1):109-17 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jan;74(1):319-23 - PubMed
    1. In Vitro. 1976 Nov;12(11):777-86 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3480-4 - PubMed

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