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. 2017 Feb;51(1):17-23.
doi: 10.1177/0023677216636832. Epub 2016 Jul 9.

Evidence for the absence of visceral pain in C57BL6/N mice subjected to therapeutically relevant O3/O2-pneumoperitoneum

Michael Bette  1 , Volker Springmann  1 , Annette Rossmann  1 , Eberhard Weihe  1 , Burkhard Schütz  1
Affiliations

Evidence for the absence of visceral pain in C57BL6/N mice subjected to therapeutically relevant O3/O2-pneumoperitoneum

Michael Bette et al. Lab Anim. 2017 Feb.

Abstract

Each different gas that is used to induce a pneumoperitoneum (PP) exhibits individual effects within the peritoneal cavity. This might include adverse effects such as pain and/or inflammatory reactions. The acute effects of ozonized oxygen (O3/O2), a highly oxidative gas mixture, after being insufflated into the peritoneal cavity are analysed in this study. Using the abdominal constriction response ('writhing') assay of chemical nociception in C57BL6/N mice, O3/O2-PP was found not to be associated with visible pain responses and did not alter the c-fos expression in the spinal cord. In addition, mRNA expression levels of the pro-inflammatory cytokines, interleukin (IL)-1β and IL-6, were found unaltered in the spleen 2 h after insufflation. In conclusion, O3/O2-PP is free of adverse pain and does not trigger inflammatory immune responses.

Keywords: cytokine; ozone; pain; pneumoperitoneum; refinement.

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