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. 2016 Feb;10(1):1-5.
doi: 10.4184/asj.2016.10.1.1. Epub 2016 Feb 16.

New Bone Formation in Tuberculous-Infected Vertebral Body Defect after Administration of Bone Marrow Stromal Cells in Rabbit Model

Ahmad Jabir Rahyussalim  1 , Tri Kurniawati  2 , Nurjati Chairani Siregar  3 , Agus Syahrurachman  4 , Ismail Hadisubroto Dilogo  1 , Diah Iskandriati  5 , Arni Diana Fitri  6
Affiliations

New Bone Formation in Tuberculous-Infected Vertebral Body Defect after Administration of Bone Marrow Stromal Cells in Rabbit Model

Ahmad Jabir Rahyussalim et al. Asian Spine J. 2016 Feb.

Abstract

Study design: Preliminary experimental study using a rabbit spondylitis model.

Purpose: To observe the ossification in a micro-environment containing live Mycobacterium tuberculosis transplanted with bone marrow stromal cells (BMSCs) in rabbits.

Overview of literature: BMSCs differentiate to osteoblasts and then osteocytes during ossification. Mycobacterium tuberculosis does not affect BMSC growth in vitro.

Methods: Six rabbits were divided into two groups of three rabbits. One group was positive for spondylitis tuberculosis by culture, polymerase chain reaction (PCR), and histopathologically. The other group was positive by PCR and histopathologically. Both groups were treated using BMSC transplantation and anti-tuberculosis drugs. After 6 weeks, ossification was evaluated by enumerating the number of osteoblasts, osteocytes, and lesion level of calcium.

Results: Mean number of osteoblasts was 207.00±31.00 in the first group and 220.33±73.46 in the second group. Mean number of intra-lesions osteocytes was in the first and second group was 18.33±30.04 and 31.00±26.87, respectively. Mean calcium level in the first group and second group was 2.94%±0.89% and 2.51%±0.13%, respectively. Total ossification score in the first and second group was 31.00 and 25.67, respectively.

Conclusions: Mycobacterium tuberculosis provides support for new bone formation by stimulating intra-lesion calcium metabolism. The microscopic environment containing live Mycobacterium tuberculosis enhances ossification.

Keywords: Calcium level; Mycobacterium tuberculosis; Ossification.

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Conflict of interest statement

Conflict of Interest: No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1. Evaluation of hemotoxylin and eosin-stained sagital section of defect tissue by microscopy at 400 times magnification. (A) Application of BMSCs in rabbit with spondylitis tuberculosis reveals inflammatory cells around the scaffold (green arrows). Osteoblast rimming (dark blue arrow) can be seen around the bony island (yellow arrow) as well as several osteocytes indicating good ossification (dark blue arrow). (B) A preparation from a rabbit that did not receive BMSCs. Few osteoblasts are evident (green arrow), with no osteocytes present. BMSCs, bone marrow stromal cells.
See this image and copyright information in PMC

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