Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Sep;15(3):888-95.
doi: 10.4314/ahs.v15i3.24.

Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens

Bolanle A Adeniyi  1 , Adewale Adetoye  1 , Funmilola A Ayeni  1
Affiliations

Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens

Bolanle A Adeniyi et al. Afr Health Sci. 2015 Sep.

Abstract

Background: The addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives.

Objective: To determine the antimicrobial activity of cow's intestinal Lactic acid bacteria (LAB) against enteric commensals.

Method: Escherichia coli, Klebsiella species (spp) and LAB were isolated from thirty different cow faecal samples and the LAB identified by partial sequencing of 16S rRNA. The antimicrobial activity of the LAB was determined against the test Escherichia coli and Klebsiella spp.

Results: Five species of LAB were isolated from thirty cow faecal samples and identified as Enterococcus hirae (8), Enterococcus durans (6), Enterococcus faecium (1), Enterococcus faecalis (1) and Weissella confusa (1). Viable cells and cell free supernatant (CFS) of the LAB were able to inhibit the growth of the test organisms with the largest zone of inhibition by the viable cells being 26mm against Escherichia coli CB6 produced by Enterococcus hirae CO6A while Weissella confusa CO29M and Enterococcus hirae CO2A produced the largest zones of inhibition (26mm) against Klebsiella CB2.

Conclusion: This study shows that LAB from cow faeces possess considerable antimicrobial activity against resistant Escherichia coli from the same environment.

Keywords: Antibacterial activities; cow faeces; enteric pathogens; lactic acid bacteria.

PubMed Disclaimer

Figures

Fig 1
Fig 1
Percentage occurrence of lactic acid bacteria isolated from cow faeces
Fig 2
Fig 2
Highest zones of inhibition by LAB against the test organisms
See this image and copyright information in PMC

References

    1. Morgavi DP, Forano E, Martin C, Newbold CJ. Microbial Ecosystem and methanogenesis in ruminants. Animal. 2010;4(7):1024–1036. - PubMed
    1. Muhammad-Lawal A, Balogun GS. Animal protein consumption among rural households in Kwara State, Nigeria. African Journal of General Agriculture. 2007;3(1):21–27.
    1. Taylor DJ. A realistic assessment of the risks of antimicrobial use in animals and its effects on food safety. Pig J. 1997;40:46–59.
    1. Khachatourians GG. Agricultural use of antibiotics and the evolution and transfer of antibiotics-resistant bacteria. Can Med Assoc J. 1998;159:1129–1136. - PMC - PubMed
    1. Food and Agriculture Organization / World Health Organization, author. Expert Workshop on Non-Human Antimicrobial Usage and Antimicrobial Resistance: Scientific assessment Geneva. 2003. Dec 1–5,

LinkOut - more resources