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. 1989 Sep;140(7):447-54.
doi: 10.1016/0923-2508(89)90065-x.

Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy

F Grimont  1 , D Chevrier, P A Grimont, M Lefevre, J L Guesdon
Affiliations
Free article

Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy

F Grimont et al. Res Microbiol. 1989 Sep.
Free article

Abstract

The use of acetylaminofluorene-labelled 16 + 23S rRNA (from Escherichia coli) is described for determining rRNA-gene-restriction patterns. The labelled probe allowed molecular fingerprinting of bacteria belonging to diverse phylogenetic branches (Enterobacteriaceae, Haemophilus, Pseudomonas, Acinetobacter, Brucella, Leptospira, Cytophaga, Campylobacter, Methylophaga). The labelled probe can be stored frozen (-20 degrees C) for at least a year and can endure vacuum dessication, ethanol precipitation or lyophilization.

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