Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
- PMID: 26965267
- DOI: 10.1007/978-1-4939-3591-8_16
Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
Abstract
Live cell-based SELEX (Systematic Evolution of Ligand EXponential enrichment) is a promising approach for identifying aptamers that can selectively bind to a cell-surface antigen or a particular target cell population. In particular, it offers a facile selection strategy for some special cell-surface proteins that are original glycosylated or heavily post-translationally modified, and are unavailable in their native/active conformation after in vitro expression and purification. In this chapter, we describe evolution of cell-type-specific RNA aptamers targeting the human CCR5 by combining the live cell-based SELEX strategy with high-throughput sequencing (HTS) and bioinformatics analysis.
Keywords: Aptamer-mediated targeted siRNA delivery; Cell-type-specific RNA aptamer; High-throughput sequencing (HTS); Human CCR5 protein; Live cell-based SELEX.
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