Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jun;101(6):e220-2.
doi: 10.3324/haematol.2015.141465. Epub 2016 Mar 11.

Bio-engineered and native red blood cells from cord blood exhibit the same metabolomic profile

Dhouha Darghouth  1 , Marie-Catherine Giarratana  2 , Lydie Oliveira  3 , Séverine Jolly  4 , Tiffany Marie  4 , Samia Boudah  5 , Nathalie Mario  6 , Christophe Junot  7 , Luc Douay  8 , Paul-Henri Romeo  3
Affiliations

Bio-engineered and native red blood cells from cord blood exhibit the same metabolomic profile

Dhouha Darghouth et al. Haematologica. 2016 Jun.
No abstract available

Keywords: RBC; bio-engineered; cord blood; metabolic profile; native.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Metabolic signatures of cRets and nRets are similar. Metabolomes of cRets from blood from 4 independent umbilical cords and nRets from 5 independent umbilical cords were analyzed by LC/MS followed by identification in the metabolite database. A diagram of high- and low-expressed metabolites is shown. cRets data are expressed as log2 fold relative to the average expression level in nRets for each metabolite. Metabolites expression was considered significantly different between cRets and nRets when log2R was above +2 or below −2 (black bars) and was considered identical when log2R was between +2 and −2 (gray bars). Statistical significance was assessed with a Mann-Whitney test: ns: not significant; *P≤ 0.05. ФThis value has been arbitrarily set at 0 (−∞ as a log20) for ease of display.
Figure 2.
Figure 2.
Clustering of metabolites expressed in cRets and nRets. (A) Shown are metabolites in equal (blue) or different (red) amounts in cRets or nRets. (B) Expanded view of metabolites expressed at different levels in cRets and nRets, and ranked according to characteristics or function. (C) Expanded view of metabolites expressed at similar levels in cRets and nRets, and ranked according to characteristics or function.
Figure 3.
Figure 3.
Metabolic signatures of red blood cells obtained by maturation of cRets and nRets. (A) Diagram of high- and low-expression metabolites in cRBCs compared to nRBCs. Black bars correspond to log2R for metabolites expressed at different levels in cRets and nRets. Gray bars correspond to log2R for metabolites expressed at different levels in cRBCs and nRBCs. Statistical significance was assessed by Mann-Whitney test: NS: not significant; *P≤ 0.05. ФThis value has been arbitrarily set at 0 (−∞ as a log20) for ease of display. (B) Metabolites differentially expressed in cRBCs and nRBCs were clustered according to function (membrane integrity or stability) or characteristic (amino acids). The number of metabolites per cluster is indicated.
See this image and copyright information in PMC

References

    1. Douay L, Andreu G. Ex vivo production of human red blood cells from hematopoietic stem cells: what is the future in transfusion? Transfus Med Rev. 2007;21(2):91–100. - PubMed
    1. Giarratana MC, Kobari L, Lapillonne H, et al. Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells. Nat Biotechnol. 2005;23(1):69–74. - PubMed
    1. Darghouth D, Koehl B, Junot C, Romeo PH. Metabolomic analysis of normal and sickle cell erythrocytes. Transfus Clin Biol. 2010;17(3):148–150. - PubMed
    1. Malleret B, Xu F, Mohandas N, et al. Significant biochemical, biophysical and metabolic diversity in circulating human cord blood reticulocytes. PLoS One. 2013;8(10):e76062. - PMC - PubMed
    1. Giarratana MC, Rouard H, Dumont A, et al. Proof of principle for transfusion of in vitro-generated red blood cells. Blood. 2011;118(19):5071–5079. - PMC - PubMed