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. 2015 Fall;6(4):279-84.
Epub 2015 Dec 15.

Genotyping of Clostridium perfringens isolated from broiler meat in northeastern of Iran

Asma Afshari  1 , Abdollah Jamshidi  2 , Jamshid Razmyar  3 , Mehrnaz Rad  4
Affiliations

Genotyping of Clostridium perfringens isolated from broiler meat in northeastern of Iran

Asma Afshari et al. Vet Res Forum. 2015 Fall.

Abstract

Clostridium perfringens (C. perfringens) is an important cause of bacterial food poisoning worldwide. The disease is caused by C. perfringens enterotoxin (CPE) encoded by cpe gene. The aim of this research was to identify the different types of C. perfringens and the presence of cpe gene in isolated bacteria from broilers' meat marketed in retail meat shops of Mashhad city in Northeastern of Iran. After isolation of C. perfringens using conventional culture method and confirmation by specific 16S rDNA gene, a multiplex polymerase chain reaction assay with specific primers, were performed for toxin typing of isolates. Clostridium perfringens was isolated from 31 broilers' meat samples (15.50%) out of 200 samples and for toxin typing the results showed 9 isolates as type A (29.03%) and 22 isolates as type C (70.96%). In this study, cpe-positive C. perfringens were detected in eight isolates of type C (25.00%). Our results indicated that C. perfringens type C is the most common type in broiler chicken carcasses.

Keywords: Clostridium perfringens; Multiplex PCR; cpe gene.

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Figures

Fig.1
Fig.1
Agarose gel electrophoresis of PCR products obtained from C. perfringens isolated from broiler carcass samples. Lane M: Marker (DNA ladder, 100 bp); C+ CIP 60.61(cpa+, cpe+) and C+ 106157 (cpa+, cpb+, etx+, cpb2+): Positive controls; C-: Negative control; Lane 4: C. perfringens type A isolate; Lanes 1, 2, 3, 5, 6, 7, 8, 9, 10: C. perfringens type C isolates
Fig. 2
Fig. 2
PCR identification of cpe gene. Lane M: Marker (DNA ladder, 100 bp); Lane 1: C+ CIP 60.61 C. perfringens (cpa+, cpb+, etx+, cpb2+) and lane 2: C+ CIP 106157: Positive controls; Lane 3 negative control; Lanes 4,5,6,7,8,9,10,11: C. perfringens type C isolates with cpe gene; Lanes 12 , 13 and 14: C. perfringens type A isolates
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