Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Published Erratum
. 2016 Mar 14;11(3):e0151886.
doi: 10.1371/journal.pone.0151886. eCollection 2016.

Correction: Curcumin Significantly Enhances Dual PI3K/Akt and mTOR Inhibitor NVP-BEZ235-Induced Apoptosis in Human Renal Carcinoma Caki Cells through Down-Regulation of p53-Dependent Bcl-2 Expression and Inhibition of Mcl-1 Protein Stability

Bo Ram Seo, Kyoung-Jin Min, Il Je Cho, Sang Chan Kim, Taeg Kyu Kwon
Published Erratum

Correction: Curcumin Significantly Enhances Dual PI3K/Akt and mTOR Inhibitor NVP-BEZ235-Induced Apoptosis in Human Renal Carcinoma Caki Cells through Down-Regulation of p53-Dependent Bcl-2 Expression and Inhibition of Mcl-1 Protein Stability

Bo Ram Seo et al. PLoS One. .
No abstract available

PubMed Disclaimer

Figures

Fig 2
Fig 2. Synergistic effect of NVP-BEZ235 and curcumin on apoptosis in Caki cells.
(A) Caki cells were treated with the indicated concentrations of baicalein, baicalin, silibinin, triptolide, kahweol, and curcumin in the absence or presence of 2 μM NVP-BEZ235 for 48 h. The sub-G1 fraction was measured by flow cytometry. (B–C) Caki cells were co-treated with 2 μM NVP-BEZ235 and 30 μM curcumin for 48 h. Cell morphology was detected by interference light microscopy (B, upper panel). The condensation and fragmentation of the nuclei were detected by 4′, 6′-diamidino-2-phenylindole staining (B, lower panel). The DNA fragmentation detection kit determined the fragmented DNA (C). Caspase activities were determined with colorimetric assays using caspase-3 DEVDase assay kits (D). (E) Caki cells were pretreated with 50 μM z-VAD-fmk (z-VAD) for 30 min, and then 2 μM NVP-BEZ235 plus 30 μM curcumin were added for 48 h. The sub-G1 fraction was measured by flow cytometry (upper panel) as an indicator of the level of apoptosis. Equal amounts of cell lysate (40 μg) were subjected to electrophoresis and analyzed by western blotting for PARP, pro-caspase 3, cleaved caspase-3 and actin as a control for protein loading (lower panel). (F) Caki cells were treated with the indicated concentrations of curcumin alone, NVP-BEZ235 alone or combined treatment with NVP-BEZ235 and curcumin for 48 h. The cell viability was assessed by XTT assay. (G) Isoboles were obtained by plotting the combined concentrations of each drug required to produce 50% cell death. The straight line connecting the IC50 values obtained for two agents when applied alone corresponds to an additivity of their independent effects. Values below this line indicate synergy, whereas values above this line indicate antagonism. The values in A, C, D, E, F, and G represent the mean ± SD from three independent samples. * p<0.001 compared to the NVP-BEZ235 alone and curcumin alone. # p<0.001 compared to the NVP-BEZ235 plus curcumin. The data represent three independent experiments.
See this image and copyright information in PMC

Erratum for

References

    1. Seo BR, Min K-j, Cho IJ, Kim SC, Kwon TK (2014) Curcumin Significantly Enhances Dual PI3K/Akt and mTOR Inhibitor NVP-BEZ235-Induced Apoptosis in Human Renal Carcinoma Caki Cells through Down-Regulation of p53-Dependent Bcl-2 Expression and Inhibition of Mcl-1 Protein Stability. PLoS ONE 9(4): e95588 doi: 10.1371/journal.pone.0095588 - DOI - PMC - PubMed

Publication types