Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jan 17;8(1):e34421.
doi: 10.5812/numonthly.34421. eCollection 2016 Jan.

Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats

Heihachi Migita  1 , Shigenori Yoshitake  1 , Yoshihiro Tange  1 , Narantsog Choijookhuu  2 , Yoshitaka Hishikawa  2
Affiliations

Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats

Heihachi Migita et al. Nephrourol Mon. .

Abstract

Background: Renal ischemia/reperfusion (I/R) injury remains a major cause of acute kidney injury (AKI), in addition to I/R injury-induced tissue inflammation, necrosis and apoptosis. Hyperbaric oxygen therapy (HBO) is defined as a treatment in which a patient is intermittently exposed to 100% oxygen pressurized to a pressure above sea level (> 2.0 atmospheres absolute (ATA), 1.0 ATA = 760 mmHg). It has been used in a number of medical conditions with a proven efficacy in a limited number of disorders. However, the effects of HBO therapy on apoptosis and proliferative activity after I/R injury have not been fully understood.

Objectives: We studied the possible beneficial effects of HBO therapy on apoptosis and tubular cell regeneration after renal I/R injury in rats.

Materials and methods: Sprague-Dawley (SD) rats were randomized into three groups: Sham (Sham-operated rats); I/R (animals submitted to I/R); and I/R + HBO (I/R rats exposed to HBO). Tubular cell apoptosis was confirmed by DNA laddering and the terminal deoxynucleotidyl transferase-mediated uridine triphosphate nick end labeling (TUNEL) assay. Cellular proliferation activity was determined using the anti-Ki-67 antibody.

Results: A significant decrease in apoptotic cells and increase in proliferative reaction were observed in the I/R + HBO group compared to the I/R group.

Conclusions: We demonstrated that HBO suppressed apoptosis, which caused inflammation after renal I/R, and promoted tubular cell regeneration. HBO has protective effects against AKI caused by renal I/R through the inhibition of apoptosis.

Keywords: Apoptosis; Hyperbaric Oxygen Therapy; Ischemia/Reperfusion Injury; Rats.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.. Effects of Hyperbaric Oxygen (HBO) on Apoptosis After Renal I/R
Sections in the cortex and the outer stripe of the medulla (OSOM) are shown at the top and bottom, respectively. A and D, issue sections obtained 24 hours after sham surgery in the cortex and OSOM, respectively.; B, E, C, F, The I/R group showed a remarkable increase in apoptotic positive nucleus (arrow in B and E) in both the cortex and the OSOM, compared to the Sham and I/R + HBO groups (C and F).
Figure 2.
Figure 2.. Number of TUNEL-Positive Nucleus in The Cortex and The Outer Stripe of The Medulla (OSOM)
TUNEL-positive nucleus number in the cortex (21.2 ± 8.5/field) and the outer stripe of the medulla (OSOM) (47.9 ± 18.0/field) were observed in the I/R group. The I/R + HBO group showed the number in the cortex (1 ± 0.1/field), OSOM (1.5 ± 0.2/field). A significant reduction in positive cells was observed in the I/R + HBO group (P < 0.01). No TUNEL positive nuclei were not found in the Sham group. ND: not detected.
Figure 3.
Figure 3.. Number of Ki-67 Antibody Positive Cells in The Cortex and The Outer Stripe of The Medulla (OSOM)
Significant increases in Ki-67 antibody positive cells in the cortex and the OSOM were observed in the I/R + HBO groups compared to the Sham and I/R groups (P < 0.01 and P < 0.05, respectively).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Sharples EJ, Patel N, Brown P, Stewart K, Mota-Philipe H, Sheaff M, et al. Erythropoietin protects the kidney against the injury and dysfunction caused by ischemia-reperfusion. J Am Soc Nephrol. 2004;15(8):2115–24. doi: 10.1097/01.ASN.0000135059.67385.5D. - DOI - PubMed
    1. Daemen MA, van 't Veer C, Denecker G, Heemskerk VH, Wolfs TG, Clauss M, et al. Inhibition of apoptosis induced by ischemia-reperfusion prevents inflammation. J Clin Invest. 1999;104(5):541–9. doi: 10.1172/JCI6974. - DOI - PMC - PubMed
    1. Lieberthal W, Triaca V, Koh JS, Pagano PJ, Levine JS. Role of superoxide in apoptosis induced by growth factor withdrawal. Am J Physiol. 1998;275(5 Pt 2):F691–702. - PubMed
    1. Wen X, Murugan R, Peng Z, Kellum JA. Pathophysiology of acute kidney injury: a new perspective. Contrib Nephrol. 2010;165:39–45. doi: 10.1159/000313743. - DOI - PubMed
    1. Rubinstein I, Abassi Z, Milman F, Ovcharenko E, Coleman R, Winaver J, et al. Hyperbaric oxygen treatment improves GFR in rats with ischaemia/reperfusion renal injury: a possible role for the antioxidant/oxidant balance in the ischaemic kidney. Nephrol Dial Transplant. 2009;24(2):428–36. doi: 10.1093/ndt/gfn511. - DOI - PMC - PubMed

LinkOut - more resources