[Simultaneous Determination of Ten Kinds of Saponins in Raw and Steamed Panax notoginseng Root and Rhizome by HPLC]

Abstract

Objective: To develop an HPLC method for simultaneous determination of notoginsenoside R₁ and ginsenosides Rg₁ Re, Rh₁, Rb₁, Rd, Rk₃, Rh₄, 20(S)-Rg₃ and 20(R)-Rg₃ in raw and steamed Panax notoginseng root and rhizome.

Methods: Vision HT C18 column (250 mm x 4.6 mm, 5 µm) was used with the mobile phase consisted of acetonitrile-H₂O in a gradient elution mode at the flow rate of 1.2 ml/min. The column temperature was maintained at 20 °C and the detection wavelength was set at 203 nm.

Results: The methodological study showed a good linear relationship ( r > 0. 9995 ) . The average recoveries of the ten saponins were 95.93%-102.54%.

Conclusion: The method is accurate and reproducible, which can be used for simultaneous determination of saponins in raw and steamed Panax notoginseng root and rhizome, and can provide reference for the relationship between material basis and pharmacological effects.