A calcium-dependent antibody for identification and purification of recombinant proteins
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A calcium-dependent antibody for identification and purification of recombinant proteins
Biotechniques.
1989 Jun.
Abstract
We report a straightforward methodology for purification of recombinant proteins by incorporating a short hydrophilic peptide marker segment at their N-termini. A calcium-dependent antibody that reacts primarily with the first three amino acids of this peptide segment was used to affinity purify the fusion proteins in a single chromatographic step. The marker peptide could subsequently be removed by proteolysis with the enzyme enterokinase.
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