Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Nov;15(3):694-6.
doi: 10.1016/j.scr.2015.10.018. Epub 2015 Nov 2.

Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF)

Kerem Fidan  1 , Gülnihal Kavaklıoğlu  1 , Ayyub Ebrahimi  1 , Can Özlü  1 , Nur Zeynep Ay  1 , Arzu Ruacan  1 , Ahmet Gül  2 , Tamer T Önder  1
Affiliations
Free article

Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF)

Kerem Fidan et al. Stem Cell Res. 2015 Nov.
Free article

Abstract

Fibroblasts from a Familial Mediterranean Fever (FMF) patient were reprogrammed with episomal vectors by using the Neon Transfection System for the generation of integration-free induced pluripotent stem cells (iPSCs). The resulting iPSC line was characterized to determine the expression of pluripotency markers, proper differentiation into three germ layers, the presence of normal chromosomal structures as well as the lack of genomic integration. A homozygous missense mutation in the MEFV gene (p.Met694Val), which lead to typical FMF phenotype, was shown to be present in the generated iPSC line.

PubMed Disclaimer

Publication types

MeSH terms