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. 2016 Jan;143(1):37-42.
doi: 10.4103/0971-5916.178587.

Cross-neutralization between three mumps viruses & mapping of haemagglutinin-neuraminidase (HN) epitopes

Sunil R Vaidya  1 , Garima M Dvivedi, Santoshkumar M Jadhav
Affiliations

Cross-neutralization between three mumps viruses & mapping of haemagglutinin-neuraminidase (HN) epitopes

Sunil R Vaidya et al. Indian J Med Res. 2016 Jan.

Abstract

Background & objectives: The reports from the countries where mumps vaccine is given as routine immunization suggest differences in mumps virus neutralizing antibody titres when tested with vaccine and wild type viruses. Such reports are unavailable from countries like India where mumps vaccine is not included in routine immunization. We, therefore, undertook this study to understand the cross-neutralization activity of Indian mumps viruses.

Methods: By using commercial mumps IgG enzyme immunoassay (EIA) and a rapid focus reduction neutralization test (FRNT), a panel of serum samples was tested. The panel consisted of 14 acute and 14 convalescent serum samples collected during a mumps outbreak and 18 archived serum samples. Two wild types (genotypes C and G) and Leningrad-Zagreb vaccine strain (genotype N) were used for the challenge experiments and FRNT titres were determined and further compared. The HN protein sequence of three mumps viruses was analyzed for the presence of key epitopes.

Results: All serum samples effectively neutralized mumps virus wild types and a vaccine strain. However, significantly lower FRNT titres were noted to wild types than to vaccine strain (P<0.05). The comparison between EIA and FRNT results revealed 95.6 per cent agreement. No amino acid changes were seen in the epitopes in the Indian wild type strains. All potential N-linked glycosylation sites were observed in Indian strains.

Interpretation & conclusions: Good cross-neutralization activity was observed for three mumps virus strains, however, higher level of FRNT titres was detected for mumps virus vaccine strain compared to Indian wild type isolates.

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Conflict of interest statement

Conflicts of Interest: None.

Figures

Figure
Figure
Deduced amino acid sequence of HN of mumps viruses isolated from India and LZ vaccine strain. Potential glycosylation sites are marked by rectangles. Regions highlighted in yellow colour indicate epitopes. Gray coloured regions are involved in viral escape from neutralizing antibodies as suggested in earlier study.
See this image and copyright information in PMC

Comment in

References

    1. Wolinsky JS, Waxham MN, Server AC. Protective effects of glycoprotein-specific monoclonal antibodies on the course of experimental mumps virus meningoencephalitis. J Virol. 1985;53:727–34. - PMC - PubMed
    1. Elango N, Varsanyi TM, Kövamees J, Norrby E. Molecular cloning and characterization of six genes, determination of gene order and intergenic sequences and leader sequence of mumps virus. J Gen Virol. 1988;69:2893–900. - PubMed
    1. Jin L, Rima B, Brown D, Orvell C, Tecle T, Afzal M, et al. Proposal for genetic characterization of wild-type mumps strains: preliminary standardization of the nomenclature. Arch Virol. 2005;150:1903–9. - PubMed
    1. World Health Organization. Mumps virus nomenclature update. Wkly Epidemiol Rec. 2012;87:217–24. - PubMed
    1. Vaidya SR, Chowdhury DT, Kumbhar NS, Tomar R, Kamble MB, Kazi MI. Circulation of two mumps virus genotypes in an unimmunized population in India. J Med Virol. 2013;85:1426–32. - PubMed

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