T1 mapping of the mouse brain following fractionated manganese administration using MP2RAGE

Abstract

With the increasing development of transgenic mouse models of neurodegenerative diseases allowing improved understanding of the underlying mechanisms of these disorders, robust quantitative mapping techniques are also needed in rodents. MP2RAGE has shown great potential for structural imaging in humans at high fields. In the present work, MP2RAGE was successfully implemented at 9.4T and 14.1T. Following fractionated injections of MnCl₂, MP2RAGE images were acquired allowing simultaneous depiction and T₁ mapping of structures in the mouse brain at both fields. In addition, T₁ maps demonstrated significant T₁ shortenings in different structures of the mouse brain (p < 0.0008 at 9.4T, p < 0.000001 at 14.1T). T₁ values recovered to the levels of saline-injected animals 1 month after the last injection except in the pituitary gland. We believe that MP2RAGE represents an important prospective translational tool for further structural MRI.

Keywords: High resolution; MEMRI; Mouse; T1 relaxation; Translational.