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Case Reports
. 2016 May 23;60(6):3821-3.
doi: 10.1128/AAC.00171-16. Print 2016 Jun.

Advanced Molecular Detection of Malarone Resistance

Eldin Talundzic  1 , Mateusz M Plucinski  2 , Shweta Biliya  3 , Luciana M Silva-Flannery  4 , Paul M Arguin  5 , Eric S Halsey  2 , John W Barnwell  5 , Fredrik Vannberg  3 , Venkatachalam Udhayakumar  5
Affiliations
Case Reports

Advanced Molecular Detection of Malarone Resistance

Eldin Talundzic et al. Antimicrob Agents Chemother. .

Abstract

The rapid emergence of drug-resistant malaria parasites during the course of an infection remains a major challenge for providing accurate treatment guidelines. This is particularly important in cases of malaria treatment failure. Using a previously well-characterized case of malaria treatment failure, we show the utility of using next-generation sequencing for early detection of the rise and selection of a previously reported atovaquone-proguanil (malarone) drug resistance-associated mutation.

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Figures

FIG 1
FIG 1
Comparison of results between (A) Sanger sequencing and (B) next-generation sequencing of D0, D11, D34, and D35 patient samples. Percentage parasitemia is shown below each day (P:n%). Percentage sequence reads contributing to the specific atovaquone-proguanil resistance-associated loci in cytochrome b are shown on the y axis, and pretreatment (D0) and day of treatment failure samples (D11 to D35) are shown on the x axis.
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