Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Feb 22;3(1):ofw031.
doi: 10.1093/ofid/ofw031. eCollection 2016 Jan.

Detection of Vaccine-Induced Antibodies to Ebola Virus in Oral Fluid

Teresa Lambe  1 , Tommy Rampling  1 , Dhan Samuel  2 , Georgina Bowyer  1 , Katie J Ewer  1 , Navin Venkatraman  1 , Matthew Edmans  1 , Steve Dicks  3 , Adrian V S Hill  1 , Richard S Tedder  2 , Sarah C Gilbert  1
Affiliations

Detection of Vaccine-Induced Antibodies to Ebola Virus in Oral Fluid

Teresa Lambe et al. Open Forum Infect Dis. .

Abstract

Blood sampling to assess production of antigen-specific antibodies after immunization is commonly performed, but it presents logistical difficulties for trials carried out during an infectious disease outbreak. In this study, we show that antibodies may be reliably detected in oral fluid collected in a minimally invasive manner without use of sharps. Clinical Trials Registration. NCT02240875.

Keywords: Ebola vaccine; antibodies; oral fluid; serology.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Comparison of antibody levels in paired oral fluid and serum from participants in the EBL001 clinical trial. (A) Immunoglobulin (Ig)M antibody levels correlated well between paired oral fluid and serum as assessed with an IgM capture assay (r = 0.78, P < .0001, 2-tailed nonparametric Spearman's correlation). (B) Immunoglobulin G antibody levels correlated well between paired oral fluid and serum as assessed with an IgG capture assay (r = 0.68, P < .0001, 2-tailed nonparametric Spearman's correlation). (C) IgG antibody levels correlated well between paired oral fluid and serum as assessed with a standardized enzyme-linked immunosorbent assay (r = 0.83, P < .0001, 2-tailed nonparametric Spearman's correlation). Dashed lines indicate assay-dependent cutoff point for sample positivity.
See this image and copyright information in PMC

References

    1. Regules JA, Beigel JH, Paolino KM et al. . A recombinant vesicular stomatitis virus Ebola vaccine - preliminary report. N Engl J Med 2015; doi:10.1056/NEJMoa1414216. - DOI - PMC - PubMed
    1. Henao-Restrepo AM, Longini IM, Egger M et al. . Efficacy and effectiveness of an rVSV-vectored vaccine expressing Ebola surface glycoprotein: interim results from the Guinea ring vaccination cluster-randomised trial. Lancet 2015; 386:857–66. - PubMed
    1. Ledgerwood JE, DeZure AD, Stanley DA et al. . Chimpanzee adenovirus vector Ebola vaccine - preliminary report. N Engl J Med 2014; doi:10.1056/NEJMoa1410863. - DOI - PubMed
    1. Rampling T, Ewer K, Bowyer G et al. . A monovalent chimpanzee adenovirus Ebola vaccine - preliminary report. N Engl J Med 2015; doi:10.1056/NEJMoa1411627. - DOI - PMC - PubMed
    1. Gilbert SC. Adenovirus-vectored Ebola vaccines. Expert Rev Vaccines 2015; 694:216–33. - PubMed

Associated data