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. 2016 Mar 9;13(3):300.
doi: 10.3390/ijerph13030300.

Biodegradation of Phenol by Bacteria Strain Acinetobacter Calcoaceticus PA Isolated from Phenolic Wastewater

Zhenghui Liu  1   2 , Wenyu Xie  3   4 , Dehao Li  5   6 , Yang Peng  7 , Zesheng Li  8 , Shusi Liu  9
Affiliations

Biodegradation of Phenol by Bacteria Strain Acinetobacter Calcoaceticus PA Isolated from Phenolic Wastewater

Zhenghui Liu et al. Int J Environ Res Public Health. .

Abstract

A phenol-degrading bacterium strain PA was successfully isolated from the effluent of petrochemical wastewater. Based on its morphological, physiological and biochemical characteristics, the strain PA was characterized as a Gram-negative, strictly aerobic, nonmotile and short rod-shaped bacterium that utilizes phenol as a sole carbon and energy source. 16S rDNA sequence analysis revealed that this strain is affiliated to Acinetobacter calcoaceticus in the group of Gammaproteobacteria. The strain was efficient in removing 91.6% of the initial 800 mg ∙ L(-1) phenol within 48 h, and had a tolerance of phenol concentration as high as 1700 mg ∙ L(-1). These results indicated that A. calcoaceticus possesses a promising potential in treating phenolic wastewater.

Keywords: Acinetobacter calcoaceticus; biodegradation; phenol-degrading.

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Figures

Figure 1
Figure 1
Transmission electron micrograph of Acinetobacter calcoaceticus PA.
Figure 2
Figure 2
Phylogenetic relationship based on the 16S rRNA gene sequences of strain PA and related organisms from the GenBank database. Bootstrap values were calculated from 1000 replications of Kimura 2-parameter, and bootstrap values higher than 50% were shown. The scale bar represents 0.002 changes per sequence position.
Figure 3
Figure 3
Profile of bacterial cell growth and phenol degradation at various initial concentrations of phenol.
Figure 4
Figure 4
Effect of pH and temperature on phenol biodegradation (initial phenol concentration was 800 mg·L−1). (a) Effect of pH on the removal of phenol; (b) effect of temperature on the removal of phenol.
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