Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013:2013:832396.
doi: 10.1155/2013/832396. Epub 2013 Jan 16.

Transplantation of Nonexpanded Adipose Stromal Vascular Fraction and Platelet-Rich Plasma for Articular Cartilage Injury Treatment in Mice Model

Phuc Van Pham  1 , Khanh Hong-Thien Bui  2 , Dat Quoc Ngo  3 , Lam Tan Khuat  1 , Ngoc Kim Phan  1
Affiliations

Transplantation of Nonexpanded Adipose Stromal Vascular Fraction and Platelet-Rich Plasma for Articular Cartilage Injury Treatment in Mice Model

Phuc Van Pham et al. J Med Eng. 2013.

Abstract

Stromal vascular fraction (SVF) combined with platelet-rich plasma (PRP) is commonly used in preclinical and clinical osteoarthritis as well as articular cartilage injury treatment. However, this therapy has not carefully evaluated the safety and the efficacy. This research aims to assess the safety and the efficacy of SVF combined with PRP transplantation. Ten samples of SVFs and PRPs from donors were used in this research. About safety, we evaluate the expression of some genes related to tumor formation such as Oct-4, Nanog, SSEA3, and SSEA4 by RT-PCR, flow cytometry, and tumor formation when injected in NOD/SCID mice. About efficacy, SVF was injected with PRP into murine joint that caused joint failure. The results showed that SVFs are negative with Oct-4, Nanog, SSEA-3, and SSEA-4, as well as they cannot cause tumors in mice. SVFs combined with PRP can improve the joint regeneration in mice. These results proved that SVFs combined with PRP transplantation is a promising therapy for articular cartilage injury treatment.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Existence of ADSCs in SVF. ADSCs were confirmed based on expression of CD44, CD105 and CD90.
Figure 2
Figure 2
Expression of Oct-3/4, Nanog, SSEA-3, and SSEA-1 in SVF. Oct-3/4 and Nanog expressed lower in embryonic stem cell (ESC) (a); while SSEA-3 and SSEA-1 did not express in SVF (b and c).
Figure 3
Figure 3
HE staining of articular cartilage. Mature cartilage layer was recorded in normal mouse (a). Mature cartilage was thinned by needle (b). Injured cartilage was regenerated in negative control group (c, e) and treated group (d, f). However, the neocartilage in treated group was thicker than in negative control group.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Prockop D. J. Marrow stromal cells as stem cells for non-hematopoietic tissues. Science. 1997;276:71–74. doi: 10.1126/science.276.5309.71. - DOI - PubMed
    1. Phadnis S. M., Joglekar M. V., Dalvi M. P., et al. Human bone marrow-derived mesenchymal cells differentiate and mature into endocrine pancreatic lineage in vivo. Cytotherapy. 2011;13(3):279–293. doi: 10.3109/14653249.2010.523108. - DOI - PubMed
    1. Estes B. T., Diekman B. O., Gimble J. M., Guilak F. Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype. Nature Protocols. 2010;5(7):1294–1311. doi: 10.1038/nprot.2010.81. - DOI - PMC - PubMed
    1. Reinisch A., Bartmann C., Rohde E., et al. Humanized system to propagate cord blood-derived multipotent mesenchymal stromal cells for clinical application. Regenerative Medicine. 2007;2(4):371–382. doi: 10.2217/17460751.2.4.371. - DOI - PubMed
    1. Phuc P. V., Nhung T. H., Loan D. T. T., Chung D. C., Ngoc P. K. Differentiating of banked human umbilical cord blood-derived mesenchymal stem cells into insulin-secreting cells. In Vitro Cellular and Developmental Biology—Animal. 2011;47(1):54–63. doi: 10.1007/s11626-010-9356-5. - DOI - PubMed