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. 2016 Mar;39(1):129-34.
doi: 10.1590/1678-4685-GMB-2015-0057.

Human mesenchymal stem cells are resistant to cytotoxic and genotoxic effects of cisplatin in vitro

Bruno Corrêa Bellagamba  1 , Bianca Regina Ribas de Abreu  2 , Ivana Grivicich  2 , Carolina Franke Markarian  3 , Eduardo Chem  3 , Melissa Camassola  2 , Nance Beyer Nardi  1 , Rafael Rodrigues Dihl  2
Affiliations

Human mesenchymal stem cells are resistant to cytotoxic and genotoxic effects of cisplatin in vitro

Bruno Corrêa Bellagamba et al. Genet Mol Biol. 2016 Mar.

Abstract

Mesenchymal stem cells (MSCs) are known for their important properties involving multilineage differentiation potential., trophic factor secretion and localization along various organs and tissues. On the dark side, MSCs play a distinguished role in tumor microenvironments by differentiating into tumor-associated fibroblasts or supporting tumor growth via distinct mechanisms. Cisplatin (CIS) is a drug widely applied in the treatment of a large number of cancers and is known for its cytotoxic and genotoxic effects, both in vitro and in vivo. Here we assessed the effects of CIS on MSCs and the ovarian cancer cell line OVCAR-3, by MTT and comet assays. Our results demonstrated the resistance of MSCs to cell death and DNA damage induction by CIS, which was not observed when OVCAR-3 cells were exposed to this drug.

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Figures

Figure 1
Figure 1. Immunophenotypic profile of cultured human adipose-derived MSCs. Cells expressed CD13, CD73 and CD90, but did not express CD69, CD117 and HLA-DR markers.
Figure 2
Figure 2. Percentage of viable cells, evaluated by MTT assay, after 72 h of exposure to increasing concentrations of cisplatin (CIS). Black bars represent MSCs and white bars correspond to OVCAR-3 cells. NC: Negative Control. *P <0.05; **P <0.01; ***P <0.001.
See this image and copyright information in PMC

References

    1. Almeida GM, Duarte TL, Steward WP, Jones GD. Detection of oxaliplatin-induced DNA crosslinks in vitro and in cancer patients using the alkaline comet assay. DNA Repair (Amst) 2006;5:219–225. - PubMed
    1. Blasiak J, Kowalik J, Malecka-Panas E, Drzewoski J, Wojewodzka M. DNA damage and repair in human lymphocytes exposed to three anticancer platinum drugs. Teratog Carcinog Mutagen. 2000;20:119–131. - PubMed
    1. Borst P, Rottenberg S, Jonkers J. How do real tumors become resistant to cisplatin? Cell Cycle. 2008;7:1353–1359. - PubMed
    1. Brozovic A, Damrot J, Tsaryk R, Helbig L, Nikolova T, Hartig C, Osmak M, Roos WP, Kaina B, Fritz G. Cisplatin sensitivity is related to late DNA damage processing and checkpoint control rather than to the early DNA damage response. Mutat Res. 2009;670:32–41. - PubMed
    1. da Silva Meirelles L, Chagastelles PC, Nardi NB. Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci. 2006;119:2204–2213. - PubMed