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Comparative Study
. 2016 Mar 24;11(3):e0151873.
doi: 10.1371/journal.pone.0151873. eCollection 2016.

Comparative Screening of Digestion Tract Toxic Genes in Proteus mirabilis

Xiaolu Shi  1   2 , Yiman Lin  2 , Yaqun Qiu  2 , Yinghui Li  2 , Min Jiang  2 , Qiongcheng Chen  2 , Yixiang Jiang  2 , Jianhui Yuan  2 , Hong Cao  1 , Qinghua Hu  2 , Shenghe Huang  1
Affiliations
Comparative Study

Comparative Screening of Digestion Tract Toxic Genes in Proteus mirabilis

Xiaolu Shi et al. PLoS One. .

Abstract

Proteus mirabilis is a common urinary tract pathogen, and may induce various inflammation symptoms. Its notorious ability to resist multiple antibiotics and to form urinary tract stones makes its treatment a long and painful process, which is further challenged by the frequent horizontal gene transferring events in P. mirabilis genomes. Three strains of P. mirabilis C02011/C04010/C04013 were isolated from a local outbreak of a food poisoning event in Shenzhen, China. Our hypothesis is that new genes may have been acquired horizontally to exert the digestion tract infection and toxicity. The functional characterization of these three genomes shows that each of them independently acquired dozens of virulent genes horizontally from the other microbial genomes. The representative strain C02011 induces the symptoms of both vomit and diarrhea, and has recently acquired a complete type IV secretion system and digestion tract toxic genes from the other bacteria.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. The dendrogram tree of six P. mirabilis strains.
Strains C05028 and C02011 are digestion tract infectious. Strains HI4320 and ATCC29906 are urinary tract infectious. Strains B02005 and C02034 are non-infectious strains.
Fig 2
Fig 2. The phylogenetic tree of the seven P. mirabilis strains based on the 16S rRNA genes.
The Cladogram tree is rooted at Proteus penneri ATCC 35198, and generated by the Phylogeny.fr. The three P. mirabilis strains inducing DTT function are highlighted in the bold font.
Fig 3
Fig 3. Venn plot of the strain specific genes in the three P. mirabilis DTT strains.
The numbers in the brackets are the numbers of DTT specific genes compared against the other P. mirabilis strains.
See this image and copyright information in PMC

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