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. 2016 Jan-Mar;10(1):92-96.
doi: 10.4103/1305-7456.175691.

Investigation of eluted monomers from resin-based root canal sealer by high-performance liquid chromatography analysis

Huma Omurlu  1 , Hacer Deniz Arisu  1 , Evrim Eliguzeloglu Dalkilic  2 , Ugur Tamer  3 , Hilal Torul  3
Affiliations

Investigation of eluted monomers from resin-based root canal sealer by high-performance liquid chromatography analysis

Huma Omurlu et al. Eur J Dent. 2016 Jan-Mar.

Abstract

Objective: The purpose of the current study was to determine the amount of urethane dimethacrylate (UDMA), bisphenol A-glycidyl methacrylate (Bis-GMA), poly (ethylene glycol) dimethacrylate (PEGDMA), bisphenol A ethoxylated dimethacrylate (Bis-EMA), and 2-hydroxyethyl methacrylate (HEMA) eluted from resin-based root canal sealer, epiphany, using high-performance liquid chromatography (HPLC).

Materials and methods: Epiphany was placed into the plastic molds and light-cured with a light emitting diode. After the curing process, each specimen in the first group (n = 12) was immersed in Eppendorf tubes containing a phosphate-buffered saline solution (PBS) and incubated for 45 s. In the second group, each specimen (n = 12) was immersed in Eppendorf tubes containing PBS and incubated for 24 h. Of the specimen extracts, 100 μL were subjected to HPLC. Analysis of data was accomplished with one-way analysis of variance (P < 0.05).

Results: All of the samples eluted HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA. A significant difference was determined between the time periods of HEMA, UDMA, PEGDMA, and Bis-EMA (P < 0.05).

Conclusion: The results of the current study showed that Epiphany releases HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA in both time periods.

Keywords: High-performance liquid chromatography; monomer; resin; sealer.

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Figures

Figure 1
Figure 1
The calibration graph for 2-hydroxyethyl methacrylate, urethane dimethacrylate and bisphenol A-glycidyl methacrylate
Figure 2
Figure 2
The calibration graph for poly (ethylene glycol) dimethacrylate and bisphenol A ethoxylated dimethacrylate
See this image and copyright information in PMC

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