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. 2016 Mar 7:7:307.
doi: 10.3389/fmicb.2016.00307. eCollection 2016.

Silver As Antibacterial toward Listeria monocytogenes

Simone Belluco  1 , Carmen Losasso  2 , Ilaria Patuzzi  3 , Laura Rigo  4 , Daniele Conficoni  4 , Federica Gallocchio  2 , Veronica Cibin  2 , Paolo Catellani  4 , Severino Segato  4 , Antonia Ricci  2
Affiliations

Silver As Antibacterial toward Listeria monocytogenes

Simone Belluco et al. Front Microbiol. .

Abstract

Listeria monocytogenes is a serious foodborne pathogen that can contaminate food during processing and can grow during food shelf-life. New types of safe and effective food contact materials embedding antimicrobial agents, like silver, can play an important role in the food industry. The present work aimed at evaluating the in vitro growth kinetics of different strains of L. monocytogenes in the presence of silver, both in its ionic and nano form. The antimicrobial effect was determined by assaying the number of culturable bacterial cells, which formed colonies after incubation in the presence of silver nanoparticles (AgNPs) or silver nitrate (AgNO3). Ionic release experiments were performed in parallel. A different reduction of bacterial viability between silver ionic and nano forms was observed, with a time delayed effect exerted by AgNPs. An association between antimicrobial activity and ions concentration was shown by both silver chemical forms, suggesting the major role of ions in the antimicrobial mode of action.

Keywords: cross-contamination; food preservation; food safety; foodborne pathogens; nanoparticles.

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Figures

FIGURE 1
FIGURE 1
Results of silver nanoparticles (AgNPs) characterization with TEM. Morphology (A) and dimensional distribution (B).
FIGURE 2
FIGURE 2
Growth curve of L. monocytogenes in the presence of 30 ppm (A), 20 ppm (B), and 10 ppm (C) AgNO3.
FIGURE 3
FIGURE 3
Growth curve of L. monocytogenes in the presence of 300 ppm AgNPs.
FIGURE 4
FIGURE 4
(A) Growth curve of L. monocytogenes in the presence of AgNO3, 30 ppm. The graphed values represents the mean of the counts belonging to Listeria monocytogenes strain n°5. (B) Time course assay of the silver ionic release of a 30 ppm solution of AgNO3, incubated in the presence of L. monocytogenes isolate 5.
FIGURE 5
FIGURE 5
(A) Growth curve of L. monocytogenes in the presence of AgNPs 300 ppm. The graphed values represents the mean of the counts belonging to L. monocytogenes strain n°5. (B) Time course assay of the silver ionic release of a 300 ppm suspension of AgNPs, incubated in the presence of L. monocytogenes isolate 5.
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References

    1. Abreu A. S., Oliveira M., de Sá A., Rodrigues R. M., Cerqueira M. A., Vicente A. A., et al. (2015). Antimicrobial nanostructured starch based films for packaging. Carbohydr. Polym. 129 127–134. 10.1016/j.carbpol.2015.04.021 - DOI - PubMed
    1. Agnihotri S., Mukherji S., Mukherji S. (2013). Immobilized silver nanoparticles enhance contact killing and show highest efficacy: elucidation of the mechanism of bactericidal action of silver. Nanoscale 5 7328–7340. 10.1039/c3nr00024a - DOI - PubMed
    1. Bae E., Park H. J., Lee J., Kim Y., Yoon J., Park K., et al. (2010). Bacterial cytotoxicity of the silver nanoparticle related to physicochemical metrics and agglomeration properties. Environ. Toxicol. Chem. 29 2154–2160. 10.1002/etc.278 - DOI - PubMed
    1. Beigmohammadi F., Peighambardoust S. H., Hesari J., Azadmard-Damirchi S., Peighambardoust S. J., Khosrowshahi N. K. (2016). Antibacterial properties of LDPE nanocomposite films in packaging of UF cheese. LWT Food Sci. Technol. 65 106–111. 10.1016/j.lwt.2015.07.059 - DOI
    1. Berton V., Montesi F., Losasso C., Facco D. R., Toffan A., Terregino C. (2015). Study of the interaction between silver nanoparticles and Salmonella as revealed by transmission electron microscopy. J. Prob. Health 03 123 10.4172/2329-8901.1000123 - DOI