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. 2016 Aug;52(4):556-60.
doi: 10.1007/s11262-016-1319-9. Epub 2016 Mar 29.

Identification and RNA segment assignment of six structural proteins of Scylla serrata reovirus

Yangyang Yuan  1   2 , Dongyang Fan  2 , Zhao Zhang  2 , Jifang Yang  2 , Jingwen Liu  3 , Jigang Chen  4
Affiliations

Identification and RNA segment assignment of six structural proteins of Scylla serrata reovirus

Yangyang Yuan et al. Virus Genes. 2016 Aug.

Abstract

Scylla serrata reovirus (SsRV) is one of the most prevalent viral pathogens of the mud crab (S. serrata). The virus represents an unassigned novel genus in the Reoviridae family, and contains 12 double-stranded RNA genomic segments. Previous analysis of virion proteins concluded that SsRV contains at least eight structural proteins, ranging from 25 to 160 kDa. Here, tandem time-of-flight mass spectrometry and Western blotting were used to re-identify the structural proteins. The results indicate that proteins encoded by SsRV segments S1, S3, S6, S9, S11, and S12 are structural proteins.

Keywords: Mass spectrometry; Reovirus; Scylla serrata; Structural proteins.

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