Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Nov 17;46(4):444-51.
doi: 10.1016/S2255-4971(15)30260-3. eCollection 2011 Jul-Aug.

PHYSICOCHEMICAL CHARACTERIZATION OF LYOPHILIZED BOVINE BONE GRAFTS

Carlos Roberto Galia  1 , André Luis Lourenço  2 , Ricardo Rosito  3 , Carlos Alberto Souza Macedo  4 , Lourdes Maria Araujo Quaresma Camargo  5
Affiliations

PHYSICOCHEMICAL CHARACTERIZATION OF LYOPHILIZED BOVINE BONE GRAFTS

Carlos Roberto Galia et al. Rev Bras Ortop. .

Abstract

To evaluate the physicochemical characteristics of lyophilized bovine grafts manufactured on a semi-industrial scale (Orthogen; Baumer S/A*) in accordance with a protocol previously developed by the authors.

Methods: The lyophilized bovine bone grafts were characterized by means of scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffractometry (XRD), thermogravimetric (TG) analysis, differential exploratory scanning calorimetry (DSC) and Fourier-transform infrared (FT-IR) spectroscopy.

Results: Ca was the main component (60%) found in the samples, followed by P (28%) and O (5%). The mean (sd) pore size was 316 μm (146.7), ranging from 91.2 to 497.8 μm, and 333.5 μm (304.8), ranging from 87.2 to 963.9 μm, at 50x and 150x magnification, respectively. The hydroxyapatite peaks were at 26°C and 32°C, and mass losses were observed between 250°C and 640°C, corresponding to organic material and water. Two temperature transitions (45.67°C and 91.89°C) showed denaturation of type 1 collagen and dehydration of hydroxyapatite.

Conclusion: The physicochemical assessment of lyophilized bovine bone grafts in accordance with the protocol developed at semi-industrial scale confirmed that this product presents excellent biocompatibility, with characteristics similar to natural bone.

Keywords: Biocompatible Materials; Bone Transplantation; General Surgery.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Images obtained via SEM on the surface of the sample: A) magnification of 50x; B) magnification of 150x; C) magnification of 500x; D) magnification of 1500x; E) magnification of 5,000x; and F) magnification of 10,000x.
Figure 2
Figure 2
Images obtained from the surface of the sample, for measuring the pore size: A) magnification of 50x; and B) magnification of 150x.
Figure 3
Figure 3
Curve obtained from the diffraction test.
Figure 4
Figure 4
Curve obtained from the thermogravimetry (TG) analysis.
Figure 5
Figure 5
Curve obtained from DSC.
Figure 6
Figure 6
FT-IR spectrum indicating the main bands observed.
See this image and copyright information in PMC

References

    1. Finkemeier CG. Bone-grafting and bone-graft substitutes. J Bone Joint Surg Am. 2002;84(3):454–464. - PubMed
    1. Laurencin CT, Khan Y. Bone grafts and bone graft substitutes: a brief history. In: Laurencin CT, editor. Bone graft substitutes. ASTM International; Bridfeport, NJ: 2003.
    1. Seiler JG 3rd, Johnson J, Hand G, Microsurgery clinic. Iliac crest autogenous bone grafting: donor site complications. J South Orthop Assoc. [periódico online]. Disponível em: http://www.medscape.com/viewarticle/410431. Acesso em: 11 setembro, 2002 - PubMed
    1. Lind M, Krarup N, Mikkelsen S, Hørlyck E. Exchange impaction allografting for femoral revision hip arthroplasty: results in 87 cases after 3.6 years' follow-up. J Arthroplasty. 2002;17(2):158–164. - PubMed
    1. Palmer SH, Gibbons CL, Athanasou NA. The pathology of bone allograft. J Bone Joint Surg Br. 1999;81(2):333–335. - PubMed

LinkOut - more resources