Immunocytochemical assays in human endometrial carcinomas: a multiparametric computerized analysis and comparison with nonmalignant changes
- PMID: 2703172
- DOI: 10.1016/0090-8258(89)90595-7
Immunocytochemical assays in human endometrial carcinomas: a multiparametric computerized analysis and comparison with nonmalignant changes
Abstract
Immunocytochemical assay (ICAs) were performed on frozen sections from human endometrial samples (n = 89) including normal endometrium, decidua, hyperplasia with and without atypia, and carcinomas. Monoclonal antiestrogen receptor (ER), anti-laminin (Lam), anti-type IV collagen (Coll IV), and anti-Ki67 were applied with avidin-biotin-peroxidase complex or peroxidase-antiperoxidase complex. The results of the ICAs were evaluated through a computerized system of image analysis referred to as SAMBA. It was shown that this system provided for an accurate reliable and reproducible analysis of ICAs in tissue sections. It is concluded that this multiparametric and standardized method of analysis of ICAs can further be applied in correlations with clinical and biochemical data.
Similar articles
-
Multiparametric evaluation (SAMBA) of growth fraction (monoclonal Ki67) in breast carcinoma tissue sections.Cancer Res. 1988 Aug 1;48(15):4368-74. Cancer Res. 1988. PMID: 3390833
-
Estrogen receptor immunocytochemical assay (ER-ICA) in human endometrium.Int J Gynecol Pathol. 1986;5(2):119-31. Int J Gynecol Pathol. 1986. PMID: 3522449
-
Correlation between laminin and type IV collagen distribution in breast carcinomas, and estrogen receptors expression, lymph node and vascular involvement.Med Oncol Tumor Pharmacother. 1990;7(1):43-54. doi: 10.1007/BF03000490. Med Oncol Tumor Pharmacother. 1990. PMID: 2187122
-
Estrogen receptor immunocytochemical assay (ERICA) and laminin detection in 130 breast carcinomas and computerized (Samba 200) multiparametric quantitative analysis on tissue sections.Bull Cancer. 1986;73(6):651-64. Bull Cancer. 1986. PMID: 3567371
-
[Endometrial cancer and steroid hormone receptors].Ugeskr Laeger. 1990 Feb 26;152(9):593-7. Ugeskr Laeger. 1990. PMID: 2408223 Review. Danish.
Cited by
-
Relationships among tenascin expression, DNA ploidy patterns, and multidrug resistance gene product (P-glycoprotein) in human colon carcinoma.Jpn J Cancer Res. 1993 Jul;84(7):703-7. doi: 10.1111/j.1349-7006.1993.tb02032.x. Jpn J Cancer Res. 1993. PMID: 7690353 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
