Isolation of a Legionella pneumophila restriction mutant with increased ability to act as a recipient in heterospecific matings
- PMID: 2703472
- PMCID: PMC209886
- DOI: 10.1128/jb.171.4.2238-2240.1989
Isolation of a Legionella pneumophila restriction mutant with increased ability to act as a recipient in heterospecific matings
Abstract
The ability of Legionella pneumophila to act as a recipient of IncP and IncQ plasmids in matings with Escherichia coli varies widely from strain to strain. We found that the low efficiency of mating of the Philadelphia-1 strain is due to a type II restriction-modification system, and we isolated and characterized a Philadelphia-1 mutant that lacks the restriction enzyme activity.
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