Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Mar 31:17:38.
doi: 10.1186/s12882-016-0252-4.

HLA-DRB1*03 as a risk factor for microalbuminuria in same duration of type 1 diabetes: a case control study

Dovilė Ražanskaitė-Virbickienė  1 , Evalda Danytė  2 , Rimantas Žalinkevičius  2
Affiliations

HLA-DRB1*03 as a risk factor for microalbuminuria in same duration of type 1 diabetes: a case control study

Dovilė Ražanskaitė-Virbickienė et al. BMC Nephrol. .

Abstract

Background: Increased urinary albumin excretion rate is the earliest clinical manifestation of diabetic nephropathy. The development of microalbuminuria in patients with type 1 diabetes mellitus (T1D) usually begins 5 to 15 years after the onset of diabetes. The rate of progression of diabetic nephropathy varies considerably among patients and not always can be explained solely by glycaemic control. The evidence suggests that genetic susceptibility may play a role in the development of diabetes microvascular complications, besides the presence of such risk factors as hyperglycaemia, hypertension, dyslipidaemia and smoking. The aim of the study was to evaluate a link between known genetic risk factors for type 1 diabetes mellitus (HLA-DR3/DR4) and microalbuminuria among patients with the same durations of diabetes.

Methods: Ninety-nine patients with T1D at the age 18-35 years were recruited for the study. The urine albumin excretion rate was normal when <30 mg/24 h; microalbuminuria 30-300 mg/24 h. Genotypes were investigated in 39 patients with normal albumin excretion rate and duration of diabetes 13.46 ± 3.72 years and in 60 patients with microalbuminuria and duration of diabetes 15.28 ± 4.08 years (p = 0.11). Genetic typing of DR3 and DR4 antigens successfully was performed for 99 subjects. Statistical analysis was performed using SPSS v. 20.0.

Results: Genotyping of 99 patients with T1D was performed: no DR3 and DR4 risk alleles were found in 22 (22.22 %) cases, DR3 alleles were present in 47 (47.48 %) cases, DR4 alleles in 25 (25.25 %) cases, and DR3/DR4 alleles in 5 (5.05 %) cases. The highest 24 h albumin excretion rate was found in patients with DRB1 gene expressed DR3 risk alleles group, the lowest - in patients with DRB1 gene with no expression of both DR3 and DR4 antigen. We confirmed the 1.87 (p = 0.021) increased relative risk for microalbuminuria in patients with DR3/DR3 alleles and same duration of diabetes. The distribution of DR3 and DR4 risk alleles was not associated with cardiovascular autonomic neuropathy both in patients with normal albumin excretion rate and microalbuminuria (1.6 vs 2.1; p = 0.21).

Conclusions: The 1.87 (p = 0.021) increased relative risk for microalbuminuria was found in patients with DR3/DR3 alleles and the same duration of diabetes.

Keywords: AER; Albuminuria; DR3; DR4; HLA; Type 1 diabetes mellitus.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Brorsson C, Hansen NT, Lage K, Bergholdt R, Brunak S, Pociot F. Identification of T1D susceptibility genes within the MHC region by combining protein interaction networks and SNP genotyping data. Diabetes Obes Metab. 2009;11(Suppl 1):60–6. doi: 10.1111/j.1463-1326.2008.01004.x. - DOI - PMC - PubMed
    1. Kazanavicius G, Barsiene L, Cibas P, Danytė E, Daugintyte-Petrosiene L, Graziene A. Endocrinology. 2009:90–97; 106–107; 167–174.
    1. Groop PH, Thomas MC, Moran JL, Waden J, Thorn LM, Makinen VP, Rosengard-Barlund M, Saraheimo M, Hietala K, Heikkila O, et al. The presence and severity of chronic kidney disease predicts all-cause mortality in type 1 diabetes. Diabetes. 2009;58(7):1651–8. - PMC - PubMed
    1. Maser RS, Lenhard MJ, DeCherney GS. Cardiovascular autonomic neuropathy: the clinical significance of its determination. Endocrinologist. 2000;10:27–33. doi: 10.1097/00019616-200010010-00006. - DOI
    1. Vergouwe Y, Soedamah-Muthu SS, Zgibor J, Chaturvedi N, Forsblom C, Snell-Bergeon JK, Maahs DM, Groop PH, Rewers M, Orchard TJ, et al. Progression to microalbuminuria in type 1 diabetes: development and validation of a prediction rule. Diabetologia. 2010;53(2):254–62. - PMC - PubMed

Publication types