Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jul 3;10(4):253-5.
doi: 10.1080/19336950.2016.1172886. Epub 2016 Apr 5.

Electrophysiological evidences of interaction between calcium channels and PA of anthrax

Evgeny Kobrinsky  1 , Nikolai M Soldatov  1
Affiliations

Electrophysiological evidences of interaction between calcium channels and PA of anthrax

Evgeny Kobrinsky et al. Channels (Austin). .
No abstract available

Keywords: Cav1.2; anthrax; calcium channel; gabapentin; patch clamp; protective antigen.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
(A) Inhibition of calcium current by PA in Cos1 cells expressing human recombinant Cav1.2 composed of ECFPN-α1C,77/β3/α2δ-1 subunits. Peak calcium currents were elicited by a stepwise depolarization to +30 mV applied from the holding potential Vh=−90 mV before (left trace) and after (right trace) application of PA-U7 (500 ng/ml, closed circles; mean ± SEM, n = 3). A 90% inhibition occurred within 5–7 min at 20–22˚C. Under the same conditions, the inactive mutant of PA, PA-3M (500 ng/ml), did not induce notable inhibition of the Ca2+ current (open circles; n = 3). Slow linear decrease of the normalized current amplitude in control is due to rundown typically seen with the Cav1.2 calcium channels (see sample Ca2+ current trace, arrow). (B) Protective effect of gabapentin. When applied at 10 µM, gabapentin inhibited ˜60% of the calcium current in 10 min, the effect reaching apparent saturation (G). Subsequent application of PA (500 ng/ml) in the presence of 10 µM gabapentin (G+PA) for 10 min did not cause a significant additional inhibition of the calcium current amplitude, suggesting protection by gabapentin against the PA-dependent inhibition of the channel. Subsequent removal of gabapentin in the presence of PA (black bar PA) did not recover the PA-dependent block of the calcium current (dotted line) measured in the absence of gabapentin (white bar PA). Mean ± SEM; n = 5.
See this image and copyright information in PMC

References

    1. Leppla SH. Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells. Proc Natl Acad Sci USA 1982; 79:3162-6; PMID:6285339; http://dx.doi.org/ 10.1073/pnas.79.10.3162 - DOI - PMC - PubMed
    1. Bradley KA, Mogridge J, Mourez M, Collier RJ, Young JAT. Identification of the cellular receptor for anthrax toxin. Nature 2001; 414:225-9; PMID:11700562; http://dx.doi.org/ 10.1038/n35101999 - DOI - PubMed
    1. Scobie HM, Rainey GJA, Bradley KA, Young JAT. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc Natl Acad Sci USA 2003; 100:5170-4; PMID:12700348; http://dx.doi.org/ 10.1073/pnas.0431098100 - DOI - PMC - PubMed
    1. Kobrinsky E, Abrahimi P, Duong SQ, Thomas S, Harry JB, Patel C, Lao QZ, Soldatov NM. Effect of Cavβ subunits on structural organization of Cav1.2 calcium channels. PLoS ONE 2009; 4:e5587; PMID:19492014; http://dx.doi.org/ 10.1371/journal.pone.0005587 - DOI - PMC - PubMed
    1. Zhang MIN, O'Neil RG. An L-type calcium channel in renal epithelial cells. Journal of Membrane Biology 1996; 154:259-66; PMID:8952955; http://dx.doi.org/ 10.1007/s002329900150 - DOI - PubMed

LinkOut - more resources