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. 2015 Jan;8(1):9-14.
doi: 10.14202/vetworld.2015.9-14. Epub 2015 Jan 2.

Seroprevalence of Mycoplasma gallisepticum antibody by ELISA and serum plate agglutination test of laying chicken

Md Zulfekar Ali  1 , Md Mostafizer Rahman  1 , Shirin Sultana  2
Affiliations

Seroprevalence of Mycoplasma gallisepticum antibody by ELISA and serum plate agglutination test of laying chicken

Md Zulfekar Ali et al. Vet World. 2015 Jan.

Abstract

Aim: Mycoplasma gallisepticum (MG) is important avian pathogens responsible for chronic respiratory diseases of chicken and turkeys, which result in large economic loss for the poultry industry. The objectives of this study were determination of seroprevalence of MG antibody of commercial layer chicken at laying period in selected areas of Bangladesh.

Materials and methods: A total of 563 blood samples were collected randomly from selected commercial layer chickens at laying period during the period from July to December, 2013. Indirect enzyme linked immunosorbent assay (iELISA) and serum plate agglutination (SPA) test were performed to detect the presence of antibodies against MG.

Results: Of 563 samples, 64.47% and 56.13% showed an overall prevalence of MG antibodies in iELISA and SPA test respectively. Prevalence of MG was recorded the highest (69.63%) at 50-55 weeks of age compared with lowest (53.26%) at 56-61 weeks of age (p<0.05). Significant (p<0.05) effect of breed were observed in the seroprevalence of MG infection in layer birds in the present study. The overall, 68.77%, 63.74% and 59.37% prevalence were found respectively in sonali, ISA Brown and White leg horn. The prevalence of MG antibodies was the highest (70.13%) in December followed by November (68%), October (65.67%), August (63.46%), September (58.54%) and July (51.78%) month. The seroprevalence of MG antibodies was higher (69.63%) in most of the large flocks and lower (56.82%) in small flocks.

Conclusion: Therefore, might be suggested that the commercial layer farms should be routinely checked to monitor MG infection and the reactor birds should be culled since MG organism has the potential to transmit vertically. The correlation between MG antibody in month and flock size was not significant (p=0.359 and p=0.868, respectively).

Keywords: Mycoplasma gallisepticum; antibodies; layer chickens; seroprevalence.

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Figures

Figure-1
Figure-1
Yellow colour develops in positive case.
Figure-2
Figure-2
Small clumps, no background clearing (+) by SPA test.
Figure-3
Figure-3
Medium sized clumps almost complete background clearing (++) by SPA test.
Figure-4
Figure-4
Large clumps, almost complete background clearing (+++) by SPA test.
Figure-5
Figure-5
Very large clumps, mostly in the periphery, complete background clearing (++++) by SPA test.
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