Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Oct;8(10):1260-5.
doi: 10.14202/vetworld.2015.1260-1265. Epub 2015 Oct 31.

Evaluation of Spirulina platensis extract as natural antivirus against foot and mouth disease virus strains (A, O, SAT2)

Hind M Daoud  1 , Eman M Soliman  2
Affiliations

Evaluation of Spirulina platensis extract as natural antivirus against foot and mouth disease virus strains (A, O, SAT2)

Hind M Daoud et al. Vet World. 2015 Oct.

Abstract

Aim: This work was aimed to document the antiviral activates of Spirulina platensis extract against foot and mouth disease virus (FMDV) different types to evaluate its replication in Baby Hamster Kidney (BHK) cell culture and in baby mice.

Materials and methods: Cytotoxicity assay studied for S. platensis extract on BHK cells to determine the non-toxic dose. The non-toxic dose of Spirulina extract was mixed with each type of FMDV (A, O, SAT2). Then 10-fold dilutions from each mixture were done. FMDV titer for each type of treated FMDV was calculated to evaluate the antiviral activity of the Spirulina extract against FMDV. Furthermore, old baby Swiss mice were inoculated with 0.1 ml intraperitonially from the mixture of FMDV different types and different concentration of Spirulina extracts. After 48 h post inoculation, all the baby mice examined to evaluate the antiviral action of Spirulina extract.

Results: The result showed that the non-toxic doses of S. platensis (50 ug/ml) revealed 35.7%, 28.5%, and 31% reductions in FMDV titers Type O, A, and SAT2 on BHK cells, respectively. The same non-toxic dose gave 50% of the inhibitory concentration in baby mice without cytotoxic effect.

Conclusion: This study confirmed the biological activity of the ethanol extract of S. platensis against FMDV Types O, A, and SAT2. From the results, S. platensis could be useful as antiviral lead to limitation of infection among animals during outbreaks but further studies need to evaluate the S. platensis on experimental or natural infected farm animals to establish the effective dose side affected period of treatment of S. platensis.

Keywords: Spirulina platensis; antiviral; foot and mouth disease virus.

PubMed Disclaimer

References

    1. Febles C.I., Arias A., Gill-Rodriguez M.C. In vitro study of antimicrobial activity in algae (Chlorophyta, Phaeophyta and Rhodophyta) collected from coast of Tenerife (in Spanish) Anu. Estud. Canarios. 1995;34:181–192.
    1. Ely R., Supriya T., NaaiK C.G. Antimicrobial activity of marine organisms collected of the coast of South East India. J. Exp. Bio. Ecol. 2004;309:121–127.
    1. Kumar V., Tirumalai P.S., Singh A., Bhatnagar A.K., Shrivastava J.N. Natural compounds from algae and S. platensis &its antimicrobial activity. Indo Glob. J. Pharm. Sci. 2013;3(3):212–223.
    1. Fujisawa T., Narikawa R., Okamoto S., Ehira S., Oshimura H., Suzuki I., Masuda T., Mochimaru M., Takaichi S., Awai K., Sekine M., Horikawa H., Yashiro I., Omata S., Takarada H., Katano Y., Kosugi H., Tanikawa S., Ohmori K., Sato N., Ikeuchi M., Fujita N., Ohmori M. Genomic structure of an economically important cyanobacterium Arthrospira (Spirulina) platensis NIES-39. DNA Res. Adv. 2010;17(2):85–103. - PMC - PubMed
    1. Karali F. Arthrospira platensis. Bio203 University of Wisconsin-La Crosse;

LinkOut - more resources