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. 2015 Dec;8(12):1400-4.
doi: 10.14202/vetworld.2015.1400-1404. Epub 2015 Dec 17.

Detection of genes mediating beta-lactamase production in isolates of enterobacteria recovered from wild pets in Saudi Arabia

Sabry A Hassan  1 , Mohammed Y Shobrak  2
Affiliations

Detection of genes mediating beta-lactamase production in isolates of enterobacteria recovered from wild pets in Saudi Arabia

Sabry A Hassan et al. Vet World. 2015 Dec.

Abstract

Aim: To determine the genetic basis and types of beta-lactamase encountered among enterobacterial isolates of wild pets from the animal exhibit.

Materials and methods: A total of 17 beta-lactamase-producing enterobacteria recovered from fecal samples of wild pet animals were analyzed for a selected beta-lactamase gene by polymerase chain reaction.

Results: Molecular analysis identified one or more β-lactamase-encoding genes in 14 enterobacterial isolates as a single or gene combination. The most frequent extended-spectrum β-lactamases types were TEM and CTX-M, and the most common AmpC enzymes were CMY-2 and DHA types.

Conclusions: The study is the first in Saudi Arabia, have established the presence of β-lactamase-encoding genes in the fecal isolates of wild pets.

Keywords: Saudi Arabia; animal exhibit; extended-spectrum β-lactamases/AmpC beta-lactamase; fecal samples; polymerase chain reaction.

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Figures

Figure-1
Figure-1
(a) The result of the multiplex polymerase chain reaction (PCR) amplification of the DNA target gene loci of 593-bp fragment DNA region coding for CTX-M; 431-bp fragment DNA region coding for TEM, (b) the result of the multiplex PCR amplification of the DNA target gene loci of: 695-bp fragment DNA region coding for CMY-2; 431-bp fragment DNA region coding for TEM; 314-bp fragment DNA region coding for DHA, (c) the result of the multiplex PCR amplification of the DNA target gene loci of 593-bp fragment DNA region coding for CTX-M.
See this image and copyright information in PMC

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