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. 2015 Nov 4;47(6):770-5.
doi: 10.1016/S2255-4971(15)30037-9. eCollection 2012.

BOVINE LYOPHILIZED GRAFT (BLG): HISTOLOGICAL ANALYSIS ON BEHAVIOR IN HUMANS AFTER 49 MONTHS

Carlos Roberto Galia  1 , Giuseppe De Luca Jr  2 , Luiz Müller Ávila  3 , Ricardo Rosito  4 , Carlos Alberto Souza Macedo  1
Affiliations

BOVINE LYOPHILIZED GRAFT (BLG): HISTOLOGICAL ANALYSIS ON BEHAVIOR IN HUMANS AFTER 49 MONTHS

Carlos Roberto Galia et al. Rev Bras Ortop. .

Abstract

Objective: To analyze the histological behavior of bovine lyophilized grafts (BLG) produced according to a protocol developed by the first author, in humans over a 49-month period by measuring the graft/bone neoformation ratio in relation to the total mineralized area.

Methods: This was a case series involving 12 patients: eight females (66%) and four males (34%), totaling 13 biopsies. BLG was used, and surgical reintervention was subsequently required during the period 2000 to 2011. The slides produced were stained with hematoxylin-eosin (HE), were analyzed by a pathologist and were digitized for the proposed evaluation.

Results: The mean age was 57 years and the mean follow-up was 49 months (range: 6-115). The average proportion of BLG was 42% (range: 13-85) and neoformed bone, 58% (range: 15-87) in relation to the total area mineralized.

Conclusions: This study demonstrated that the BLG used presented osteoconductive characteristics and biocompatibility. BLG is a therapeutic option that can be used in orthopedic surgery in which bone defects need to be filled.

Keywords: Bone Transplantation; Freeze Drying; Human.

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Figures

Figure 1
Figure 1
Lyophilized bovine graft (LBG) stained blue with hematoxylin-eosin (HE). 10x magnification. HCPA 2011.
Figure 2
Figure 2
Blue areas denote the lyophilized bovine graft (LBG) stained with hematoxylin-eosin (HE). 100x magnification. HCPA 2011.
Figure 3
Figure 3
Fibrosis represented by whitish light areas in the center of the image. 200x magnification. HCPA 2011.
Figure 4
Figure 4
Lyophilized bovine graft (LBG) (blue areas) surrounded by newly formed bone tissue (pink areas). 200x magnification. HCPA 2011.
Figure 5
Figure 5
Partially resorbed (blue areas) of lyophilized bovine graft (LBG) surrounded by newly formed bone tissue (pink areas). 200x magnification. HCPA 2011.
Figure 6
Figure 6
Well-defined boundaries between the bluish lyophilized bovine graft (LBG) and the pink new bone. 400x magnification. HCPA 2011.
See this image and copyright information in PMC

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