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. 2015 Dec;57(6):537-9.
doi: 10.1590/S0036-46652015000600015.

MOLECULAR IDENTIFICATION OF Pseudoterranova azarasi LARVAE IN COD (Gadus sp.) SOLD FOR HUMAN CONSUMPTION IN BRAZIL

Juliana Marigo  1 , Sueli Akemi Taniwaki  2 , Pedro Luiz Silva Pinto  3 , Rodrigo Martins Soares  2 , José Luiz Catão-Dias  1
Affiliations

MOLECULAR IDENTIFICATION OF Pseudoterranova azarasi LARVAE IN COD (Gadus sp.) SOLD FOR HUMAN CONSUMPTION IN BRAZIL

Juliana Marigo et al. Rev Inst Med Trop Sao Paulo. 2015 Dec.

Abstract
in English, Portuguese

Anisakiasis and Pseudoterranovosis are human diseases caused by the ingestion of live Anisakidae larvae in raw, undercooked or lightly marinated fish. Larvae were collected from one salted cod sold for human consumption in a Sao Paulo market in 2013. One section of one brownish larva was used for molecular analyses. The partial COX2 gene sequence from the larva had a nucleotide identity of 99.8 % with Pseudoterranova azarasi, which belongs to the Pseudoterranova decipiens species complex. The risk of allergy when consuming dead larvae in salted fish is not well known and should be considered.

Os termos Anisakiasis e Pseudoterranovosis são utilizados para doença em humanos causada pela ingestão de larvas vivas de parasitas da Família Anisakidae em peixes crus, mal cozidos ou levemente marinados. As larvas foram coletadas de bacalhau salgado vendido para consumo humano num mercado de São Paulo em 2013. Uma parte da larva de cor castanha foi utilizada em análises moleculares. A sequencia parcial do gene COX2 obtida da larva mostrou 99,8% de identidade de nucleotídeos com Pseudoterranova azarasi, que faz parte do complexo de espécies Pseudoterranova decipiens. O risco de reação alérgica envolvido no consumo de larvas mortas em peixe salgado não é bem conhecido e deve ser considerado.

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Figures

Fig. 1
Fig. 1. - Phylogenetic tree of cytochrome oxidase subunit II (COX2) gene constructed by Neighbor-Joining method using the Maximum Composite Likelihood approach (superior bootstrap value) and Maximum Likelihood method based on the Hasegawa-Kishino-Yano model (inferior bootstrap value). Only bootstrap values superior to 70 are shown at the nodes. Anisakis nascettii was used as an out-group, the sequence in bold indicates the one from this study.
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References

    1. Amato V, Neto, Amato JG, Amato VS. Probable recognition of human anisakiasis in Brazil. Rev Inst Med Trop Sao Paulo. 2007;49:261–262. - PubMed
    1. Arizono N, Miura T, Yamada M, Tegoshi T, Onishi K. Human infection with Pseudoterranova azarasi roundworm. Emerg Infect Dis. 2011;17:555–556. - PMC - PubMed
    1. Armentia A, Martín-Gil FJ, Pascual C, Martín-Esteban M, Callejo A, Martínez C. Anisakis simplex allergy after eating chicken meat. J Investig Allergol Clin Immunol. 2006;16:258–263. - PubMed
    1. Audicana MT, Kennedy MW. Anisakis simplex: from obscure infectious worm to inducer of immune hypersensitivity. Clin Microbiol Rev. 2008;2:360–379. - PMC - PubMed
    1. Brasil. Ministério da Agricultura . Regulamento da inspeção industrial e sanitária de produtos de origem animal (aprovado pelo Decreto no 30.691, de 29-3-1952, alterado pelo Decreto no 1.255, de 25-6-1962) Brasília: Ministério da Agricultura; 1980. 74-9

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