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. 1997 Dec 15;3(4):225-7.
doi: 10.3748/wjg.v3.i4.225.

Plasma D (-)-lactate as a new marker for diagnosis of acute intestinal injury following ischemia-reperfusion

Y M Yao  1 , Y Yu  1 , Y Wu  1 , L R Lu  1 , Z Y Sheng  1
Affiliations

Plasma D (-)-lactate as a new marker for diagnosis of acute intestinal injury following ischemia-reperfusion

Y M Yao et al. World J Gastroenterol. .

Abstract

Aim: To observe the kinetics of D (-)-lactate alteration in both portal and systemic circulation systems, and its relationship with intestinal injury in rats subjected to acute intestinal ischemia-reperfusion.

Methods: Anesthetized rats underwent a 75-min superior mesenteric artery occlusion followed by a 6-h reperfusion. Plasma D (-)-lactate levels were measured by an enzymatic spectrophotometric assay.

Results: Intestinal ischemia for 75 min resulted in a significant elevation of D (-)-lactate levels in the portal vein, as compared with the baseline values (P < 0.05). Plasma D (-)-lactate levels had a tendency to further increase after reperfusion, up to 6 h. Similar alterations in D (-)-lactate were also found in systemic circulation, and there were no significant differences between the portal and systemic circulations at any time point. Moreover, the macropathological evaluation scores were significantly correlated to the portal D (-)-lactate levels in animals at various time points (r = 0.415, P < 0.01). In addition, there was a remarkable rise of endotoxin concentration within the portal vein at the end of the 75-min ischemic period (P < 0.05), reaching a peak at 2 h post-reperfusion.

Conclusion: Acute intestinal ischemia is associated with failure of the mucosal barrier resulting in increased plasma D (-)-lactate levels in both portal and systemic blood. The subsequent reperfusion might further increase D (-)-lactate levels, which are correlated to the macropathological alterations. Plasma D (-)-lactate may be a useful marker of intestinal injury following both ischemia and reperfusion insults.

Keywords: D (-)lactate; Endotoxin; Intestinal injury; Reperfusion injury.

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Figures

Figure 1
Figure 1
Kinetics of plasma D (-)-lactate alteration in portal and systemic circulation systems. Six to nine animals were assessed for each time point. Data are expressed as x ± sx-. aP < 0.05 and bP < 0.01 vs baseline values.
Figure 2
Figure 2
Correlation between the macropathological evaluation scores of intestine and the portal D (-)-lactate levels in animals subjected to intestinal ischemia-reperfusion injury.
Figure 3
Figure 3
Changes in plasma endotoxin concentration in portal vein following acute intestinal ischemia-reperfusion injury. Six to nine animals were assessed for each time point. Data are expressed as x ± sx-. aP < 0.05 and bP < 0.01 vs baseline values.
See this image and copyright information in PMC

References

    1. Swank GM, Deitch EA. Role of the gut in multiple organ failure: bacterial translocation and permeability changes. World J Surg. 1996;20:411–417. - PubMed
    1. Yao YM, Bahrami S, Leichtfried G, Redl H, Schlag G. Pathogenesis of hemorrhage-induced bacteria/endotoxin translocation in rats. Effects of recombinant bactericidal/permeability-increasing protein. Ann Surg. 1995;221:398–405. - PMC - PubMed
    1. Yao YM, Sheng ZY, Tian HM, Wang YP, Yu Y, Fu XB, Lu LR, Wang DW, Ma YY. Gut-derived endotoxemia and multiple system organ failure following gunshot wounds combined with hemorrhagic shock: an experimental study in the dog. J Trauma. 1995;38:742–746. - PubMed
    1. Smith SM, Eng RH, Buccini F. Use of D-lactic acid measurements in the diagnosis of bacterial infections. J Infect Dis. 1986;154:658–664. - PubMed
    1. Murray MJ, Barbose JJ, Cobb CF. Serum D(-)-lactate levels as a predictor of acute intestinal ischemia in a rat model. J Surg Res. 1993;54:507–509. - PubMed

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