Determination of β-glucuronidase in human colorectal carcinoma cell lines
- PMID: 27053883
- PMCID: PMC4806251
- DOI: 10.3748/wjg.v3.i4.251
Determination of β-glucuronidase in human colorectal carcinoma cell lines
Abstract
Aim: To study the relationship between β-glucuronidase and the invasiveness of human colorectal carcinoma cell lines.
Methods: Six colorectal carcinoma cell lines, including three well-differentiated (CX1, CCL187, and CCL229) and three poorly differentiated ones (CCL227, CCL228, and Clone A), were analyzed by Fischman's method to determine the concentration of β-glucuronidase in the medium.
Results: Low levels of β-glucuronidase (activity range: 1.29 to 1.96 μg/106 cells·h) were associated with poor invasiveness. This finding was in contrast to the elevated levels of the enzyme (2.46-3.37 μg/106·h) detected in the medium derived from the more aggressively invasive cells (CCL 227, CCL 228, Clone A, and CCL 229).
Conclusion: Highly invasive colorectal carcinoma cells secreted higher levels of β-glucuronidase than the poorly invasive cells. Determination of secreted β-glucuronidase might represent a useful in vitro measurement tool to assess the invasiveness of colorectal carcinoma.
Keywords: Cell lines; Colorectal neoplasms; β-glucuronidase invasiveness.
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